| Adhesion molecules (AMs) are glycoproteins expressed by endothelial cells, white blood cells and other cells. They are important mediators of cell-cell interaction, and regulate cell fate determination by influencing growth, differentiation and organization tissues. They also involve in physiologic process and physiopathologic process such as immune response. AMs expressed in the cells' surfaces become soluble adhesion molecules when they fall into the blood. Soluble adhesion molecules make it possible for people to determine AMs. AMs's expression is up-regulated in autoimmune disease, hypertension, hyperlipemia, thyroid disease and so on. It had been discovered that AMs play an important role in the molecular mechanism of beta-cell destruction in type 1 diabetes mellitus (DM). The relationship between AMs and microvascular complication in type 1 DM was also be emphasized. These works on type 2 DM are few, and some results were effected by hypertension and hyperlipemia. E-selectin and vascular cell adhesion molecule-1 (VCAM-1) are AMs which relate with DM mostly. The aim of this study was to investigate the changes and to assess the significance of serum soluble E-selectin (sE-selectin) , soluble VCAM-1 (sVCAM-1) in patients with type 2 DM.Patients and methods42 patients diagnosed with type 2 DM according to ADA criteria and20 age and sex matched healthy controls were included in our study. The diabetic group comprised 27 men and 15 women (average age 60.74 ?12.14years , average duration 6.88+5.82 years). No patient had hypertension, hyperlipemia, ketonemia, acute and chronic infectious diseases. All patients were receiving oral hypoglycemic agents at the time of study and had no insulin injection history. The diabetic group was divided into two subgroups: DM with diabetic microvascular complication ( retinopathy or/and nephropathy ) subgroup (9 men , 9 women, average age 63.67 ?11.99years , average duration 9.83+5.49 years) and DM without diabetic microvascular complication subgroup (17 men, 7 women, average age 56.79 ?11.82 years, average duration 2.94+3.48 years) . Fasting serum sE-seletin and sVCAM-1 levels were determined in all subjects by enzyme-linked immunosorbent assay. Fasting blood glucose (FBG), triglyceride (TG), and low-density lipoprotein cholesterin (LDL-C) levels were also measured in all subjects. Fasting plasma HbAjC level was determined in all diabetic patients by immunonephelometry assay. The statistical ways were t test , x 2 test, and correlation test.ResultsThe duration of DM with diabetic microvascular complication subgroup was longer than that of DM without diabetic microvascular complication subgroup (t=4.66, P<0.01). TG and LDL-C levels had no significant difference in every group (P>0.05). FBG level in diabetic group, in DM with diabetic microvascular complication subgroup, in DM without diabetic microvascular complication subgroup and in control group was 10.19?.10 mmol/1, 10.79?.74 mmol/1, 9.39+4.52 mmol/1 and 4.91?.49 mmol/1 respectively. FBG levels in the former 3 groups were higher than that in the control group (P<0.01). There was no significant difference in FBG and HbA1c levels between DM with diabetic microvascular complication subgroup and DM without diabetic microvascular complication subgroup (t=1.101, 1.032, P=0.278, 0.313 ).Serum sE-selectin levels ( 84,78 ?49.22 ng/ml vs 39.42 ?18.11 ng/ml ) and sVCAM-1 levels ( 510.14 ?159.02 ng/ml vs 400.60 + 67,52 ng/ml ) were significantly increased in diabetics compared with controls ( p< 0.01 ). Serum sE-selectin levels ( 103.19 ?9.81 ng/ml vs 70.96 ?34.29 ng/ml ) and sVCAM-1 levels ( 613.17 ?155.66 ng/ml vs 432.87 ?112.38 ng/ml) were higher in DM with diabetic microvascular complication subgroup than those in DM without diabetic microvascular complication subgroup (p<0.05). Serum sVCAM-1 level was no significant difference between DM without diabetic microvascular complication subgroup and control group (t=1.125, P>0.05).Serum sE-selectin level was positively correlated with plasma...
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