| Neural cell adhesion molecule L1 (LI), discovered in the 80s, is one of the matters that can promote nerve regeneration. It can mediate the interaction of cell-to-cell and cell-to-matrix, take great efforts on neurite outgrowth and fasciculation, migration of neural cells, formation of the myelin, structure of neural trace, transmembrane signal transduction, inhibiting apoptosis of neural cells, even on immune system and formation of the tumor. Li is discovered on the mouse's Cerebellar membrane primarily. Thereafter, it also is found in post- mitotic neurons, pre- and non-myelinating Schwann cells and so on. The studies of the L1 are usually concentrated on cell biology and molecule biology at the past. The experiments in animal bodies are done relatively scarcely. The purpose of this experiment is to observe the function of Li which is derived from Schwann cells to damaged spinal cord by morphology, electrophysiology, immunohistochemisty, to explore a new feasible method to spinal cord injury, and to provide a reliable experimental datum for its clinical use in future. The main contents and conclusions of this experiment are as follows: I . Culture and immunohistochemistry of Schwann cells Schwann cells that are derived from sciatic nerve of Sprague-Dauley rats are successfully isolated, cultured. The Schwann cells are transplanted into the hemisected adult rat spinal cord after purification and condense and enclosed by L I -antibody. Eight weeks later, the experiments of the S- 100 immunohistochemistry indicate that Schwann cells are still alive and array orderly relatively. 2 Make mode The animal models of the hemisected spinal cord are built up successfully. The outcome of HE staining indicates that the other side of the adult spinal cord is kept very well. 3 According to the norm of developed Rivlin test and BBB locomotor score, the observations of the actual function of rats are done on the condition that the rats are given different manage. Conclusions show that the animals that are transplanted by the Schwann cells have the best functional recovery of all animals. The using of L 1 antibody descend the functions, But they are still better than the controls. 4~ The electrophysiological techniques of SEP and MEP are used to study the transduction of neural impulse. The results of the experiment indicate the transplantation of the Schwann cells takes benefits of damaged spinal cords. The latency of the waves of SEP or MEP is extracted. But it still is the best in all. The control group is the worst of all. The wave can be found again after the spinal cords are hemisectcd in control group. 5.. Seen from the immunohistochemistry of NF, Schwann cells can promote neural dendrite outgrowth toward the area of hemisected spinal cord. Once the LI antibody is applied, the length of the regenerated neural dendrite will be short. It is means that the capability of dendrite outgrowth will decline. 6.. HRP can be absorbed by neural cells and transported in cells. The technique of HRP tracing can show neural fibers directly. Seen from the outcome, the neural fibers of the group of using Li which extend over the area of spinal cord injury are less than that of the group of transplanting Schwann cells. However, the control group is been able to find no fibre gr...
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