Growth Characteristic Of Both Rat Neural Stem Cells And Schwann Cells Co-cultured In Vitro And Their Combined Transplantation Into Injured Spinal Cord

The incidence of central nerve system diseases is increasing along with the increase of people's life expectancy and the social develompment. For a variety of injuries , aging , the incidence of spinal traumatism and others. The regeneration of damaged neurons is almost impossible under natural history or traditional operations and druggery therapy because of the physiological speciality of CNS, ie its poor regeneration as well as renovation ability. What hyperplastic cell is only glial cell forming glial scar. There are phenomena of partial losses in structure and function. Even there are partial resume function, they are only due to neuronal plasticity. They are limited in number and can not replace injuries completely. The newly formed glial scar often block the reorganization and going through of neural fibers. These pathological characteristics are puzzling difficult problem points and theraputic effects of CNS injuries are not satisfactory. With the finding of blood-building stem cells, medical scientists from many countries are committing themselves to research whether NSC exists. In recent ten years, it has been reported that CNS of adult mammal animals, including human beings, has some NSC which have potentials of mitosis and differentiation to some extent.This experiment is taking advantage of the Sc which can secret neurotrophic factors(such as Laminin). Induce neural axon formation, prolong, restrain formation of glial scar and help injured neurons to repair, reproduc in structure as well as to reconnect in functional transduction. NSC have potential of differentiation into manydirections and can be translated into neurons when transplanted into the field of spinal injury. The combined transplantation of both Sc and NSC can rebuild the structure and function of CNS.We will testify the superiority of the combined transplantation in vivo and vitro. Dissociated, purify and amplify NSC and Sc respectively, incubate them together to observe Sc's effect on the transformation and growth of NSC, then inject them into the injurying field to see the recovering status of spinal injuries. Results:(1) In vitro, Sc improve NSC to transform into neurons and it also can improve the growth of neural axon. (2) When transplant Sc and NSC into rat's spinal injuried field, we can observe living cells, emigration and a great deal of neurons. The rats recover some functions of feeling and movement in their paralyzed part of the bodies. (3) We set up a kind of improved rat model of hemisection spinal cord in rats, improve hemisection spinal cord pattern, and make operations simpler with increased hemisection spinal cord and efficiency.In total, We utilized physiological character of these two kinds cells in cell biology in the experiment which improve effective regeneration and repair of neuro cells. This method of combined transplantation not only can compensate lost neurons, bue also can protect living neurons, and improve the prolong of neural axon, increase the rate of neural reconnection and livability as well as enhance their intercommunication. It is more applicable clinically and will lay a concrete foundation theoretically and experimentally for the treatment of human nerve system diseases esp spinal injury.