Nutritional Therapy Of Experimental Nephrotic Syndrome With Soybean Protein And Isoflavones And Nutritional Status Assessment Of Patients With Nephritic Syndrome

Part l:The effect of nutritional therapy in NS rats with different kinds of protein Objective: To investigate the different therapeutical effect of soy protein and casein in experimental NS rats. Method: A model of nephrotic syndrome rats was induced after tail intravenous injections with ADR (5.0mg/kg). 40 rats were randomly divided into five groups: Group A were treated with 100% soybean protein; Group B were treated with 50% soybean protein and 50% casein ; Group C with 25% soybean and 75% casein and group D with casein. Group E were treated with 0.9% NaC1 as normal control. After being collected urine and determined the content of urine protein, all the rats were killed and taken blood and kidneys 30 days later. several indexes was examined ,such as serum biochemical indexes(TG,Tch,TP,ALB,HDL LDL ), kidney function indexes(UN. Cr) , kidney antioxidant indexes (SOD,MDA,GSH-Px, GSH) and the changes of kidney by light microscopy and electron microscopy. Results: Hyperlipidemia,Hypoalbuminemia and proteinuria were observed in NS rats .TP,ALB,A/G decreased and TG,Tch,HDL,LDL increased compared with those in group E(P<0.05). In group A, urine protein was lower than that in group D(P<0.0 1). A/G in group A,B and C were higher than that in group D(P<0.05). TG in group A was lower than that in group D (P<0.0 1). HDL in group A was higher than that in group D.LDL in group A and B were low than that in group D. UN in All NS groups were higher than that in group D (P<0.05). Serum Fe in group K. B and C were higher than that in group D(P<0.0 1). Morphologic indexes by electronmicroscopy noted widespread fusion of foot processes and activation of podocytes,loss of foot process architecture .Group A were better than grouP D. SOD. MDA.GSH-Px and GSH indicated that SOD and MDA had no obvious difference in allgroups. GSH-Px in group D was higher than those in grouP A. B and C(P<0.0l).MDA in group D was higher than that in group A. B and C.ConcIusion: Soybean protein can improve the nutritional statlls of NS and increasethe anioxidan ability of body, protect kidney function.Part 2 The nutritional therapy of experimental nephrotic syndrome withi sofl avonesObjective: To study the nutrition therapelltical effect of isoflavones in rats withadriamycin-induced nephrotic syndrome (NS).Method: A model of nephrotic syndrome rats was induced after tail ifitfavenousinjections (5.0mg/kg). 32 rats were randomly divided into four grouPs: GrouP A weretreated with soybean protein which coniain isoflavones 3.4 mg /g protein, Rats ingrouP B were treated with soybean protein without isoflavones and group C withcasein. Group D were treated with 0.9% NaCl as normal control .several indexes wasexamined:serum biochemical indexes (TG. Tch. TP. ALB. HDL. LDL'Ca' Fe),urine protein,kidney function indexes(UN. Cr),kidney anioxidant indexes(SOD' MDA. GSH-Px. GSH), kidney tissue examination by light microscopy \electron microscopy and the positive rate of TGF-P, in kidney examined byimmtmohistochemistry.Results: Hyperipidemia. Hypoalbuminemia and proteinuria were observed inexperimental NS rats. TP. ALB decreased and TG. Tch increased compared withthose in group D(P<0.05). In grouP A, Tch and TG were lower than those in grouP C.There is no markedly difference for serum TP in all NS groups. All the NS rats wereobserved the injury of kidney function. But UN in group A was better than that ingrouP C. SOD. MDA. GSH-Px and GSH indicated that grouP C was the best in NSrats. Morphologic indexes by electron micro5copy noted widespread fusion of fOotprocesses and activation of podocytes, loss of fOot process architectate .GrouP Awere better than group A. B and C. The positive rate of...