| [Objective] 1. To observe the combined effects of soybean isoflavones andvinorelbine on the models of VX2 cancerous tissue transplantable subcutaneous tumorin rabbit and explore the relations between the effects and imbalance of oxidation andanti-oxidation. 2. To establish the models of VX2 cancerous cell transplantablepulmonary tumor in rabbit and observe the relations between some of biologicalspecialties of the models and the rabbit VX2 transplantable pulmonary tumors.[Methods] 1. Eighteen Japanese white rabbits were inoculated 2.0 ml suspensionconcentrating VX2 cancerous tissue on the right back leg of the animals respectivelyvia subcutaneous injection to establish the models of VX2 cancerous tissuetransplantable subcutaneous tumor. All rabbits were randomly divided into threegroups after the models were established. A- group(control group): Six experimentalrabbits were fed with only general rabbit feed and water. B-group(vinorelbine-treatedgroup): Six experimental rabbits were fed with general rabbit feed and water. Theserabbits were injected 2.5 mg vinorelbine on the fifteenth day and 2.0 mg vinorelbineon the twenty-second day after inoculating VX2 cancerous tissue suspension via veinrespectively. C-group(soybean isoflavones and vinorelbine-treated group): Sixexperimental rabbits of this group received the same treatment as the rabbits of groupB, but these rabbits were fed 50 mg/kg soybean isoflavones everyday respectivelyfrom the eighth day to the thirtieth day after inoculating VX2 cancerous tissuesuspension. All the VX2 cancerous tissue transplantable subcutaneous tumors weremeasured and compared the tumor growth rates once weekly. All experimental rabbitswere draw blood-plasma and measured livingness of SOD and content of MDA, and killed these animals to observe transferable cancerous focus on the thirtieth day afterinoculating VX2 cancerous tissue suspension. 2. The VX2 cancerous cell suspensionwas made. Six Japanese white rabbits were inoculated to the fight-lung with 0.1 mlsuspension concentrating 1×10~7/ml of alive cells respectively. To observe the successrate of establishing VX2 cancerous cell transplantable pulmonary tumor models andthe survivable time of these experimental rabbits and measure expression of bcl-2protein and bax protein in VX2 cancerous cell transplantable pulmonary tumors byimmuneeohistochemical analysis.[Results] 1. The success rate of establishing VX2 cancerous tissue transplantablesubcutaneous tumor models is 100%. These transplantable subcutaneous tumor didn'ttransfer to other organs of the experimental rabbits. 2. The growth rate oftransplantable subcutaneous tumor in C-group were significantly lower as comparedwith A-group before the experimental rabbits of C- group received vinorelbine-treated,P＜0.01. After the experimental rabbits of B-group and C-group receivedvinorelbine-treated, the growth rate of transplantable subcutaneous tumor in B-groupwere lower than A group, P＜0.05; the growth rate of transplantable subcutaneoustumor in C-group were lower than B-group, P＜0.05, and significantly lower thanA-group, P＜0.01. After the experimental rabbits of B-group and C-group receivedthe second vinorelbine-treated, the growth rate of transplantable subcutaneous tumorin B-group and C-group were significantly lower than A group, P＜0.01; the growthrate of transplantable subcutaneous tumor in C-group were lower than B-group, butthere was not statistically significant difference, P＞0.05. 3. Content of MDA inplasma of the experimental rabbits: B-group significantly more than A-group, P＜0.01;C-group was less than B-group, P＜0.05. Livingness of SOD in plasma of theexperimental rabbits: B-group significantly less than A-group, P＜0.01; C-groupwere more than B-group, P＜0.05. 4. The success rate of establishing VX2cancerous cell transplantable pulmonary tumor models was 83.33%(5/6), the meansurvivable time of the experimental rabbits bearing transplantable pulmonary tumorwas 35.4±3.78 days. These transplantable pulmonary tumors were able to transplantand grow on surrounding and distant organs. 5. Expression of bcl-2 protein in VX2cancerous cell transplantable pulmonary tumors were 80%(4/5); expression of Bax protein in VX2 cancerous cell transplantable pulmonary tumors were 20%(1/5), therewas not statistically significant difference in the expression rate of between Bcl-2protein and Bax protein in VX2 cancerous cell transplantable pulmonary tumors, P＞0.05.[Conclusion] 1. Soybean isoflavones could inhibit the growth of rabbit-VX2cancerous tissue transplantable subcutaneous tumor when the experimental rabbitswere fed 50 mg/kg soybean isoflavones everyday respectively from the eighth day tothe thirtieth day after inoculating VX2 cancerous tissue suspension. 2. The combinedeffects of soybean isoflavones and vinorelbine were inhibit cooperatively the growthof rabbit-VX2 cancerous tissue transplantable subcutaneous tumor when theexperimental rabbits were fed 50 mg/kg soybean isoflavones everyday respectivelyfrom the eighth day to the thirtieth day after inoculating VX2 cancerous tissuesuspension. 3. The experimental rabbits receiving vinorelbine-treated would catchpneumonia because of hematological influence of vinorelbine, and which suggest thatthem was antioxidant injury to the experimental rabbits because of pneumonia.Soybean isoflavones could increase the antioxidant ability of these experimentalrabbits, and reduce injury of the imbalance of oxidation and anti-oxidation. 4. Themodel of VX2 cancerous cell transplantable pulmonary tumors in rabbit wasestablished, but the success rate wasn't 100%, the experimental methods should beimproved in future.
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