| Glioma is the most common central nervous system malignant tumor. Even though the patients can receive synthetical therapies, they get poor outcome. The ultimate symbol of cancer is unlimited independent division and proliferating of cell, which need to be completed by cell cycle. Blocking or relaxing cell cycle through regulating the controlling factors of cell cycle would be a breakthrough of cancer genetherapy. As a new cyclin dependent kinase inhibitor, p27 strongly inhibits cell cycle by many mechanisms and is known as a cancer suppressor. But p27 gene was rarely changed in many malignant tumors. The expression of p27 protein was low in cancer and was related to oncogeriesis, malignant degree of tumors and outcome of patients. Cyclin A promotes the transforming of cell cycle and involves in tumorigenesis. We studied p27, p27mRNA and cyclin A expression and their relationship with cellular proliferating activity (proliferating cell nuder antigen labelling indices, PCNALI) in human brain gliomas. Part one: p27, cyclin A and PCNA expression in human brain gliomas Immunohistochemical technique was used to detect the expression of p27, cyclin A and PCNA in gliomas and normal brain tissue (NBT). The positive rate and mean labelling indices for p27 in NBT were 100% (5/5) and 57.36? 19.4 respectively, those in gliomas were 64.3% (27/42) and 17.55 ?.72 respectively. Contrast to NBT, the expression of p27 in gliomas reduced remarkablely (P<0.0l) . With the increasing of malignancy scale, positive rate and MU for p27 decreased gradually (P<0.O1). There was a reverse relationship between the labelling indices for p27 and those for PCNA (r=-O.632, P<0.01) Cyclin A was not expressed in NBT. The positive rate and mean labelling indices for cyclin A in gliomas were 62% (26/4Z) and 4.05 ?5.32 respectively. With the increasing of malignancy scale, positive rate and mean labelling indices for cyclin A increased gradually (P |
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