| Proton Magnetic Resonance Spectroscopy Study on Cerebral Metabolism before and after Infarction inRatsAbstractBackground and Purpose:Cerebrovascular disease is one of main diseases that make people dead or unable. It's also the number one killer in China. Cerebral infarction takes up 75% of all the cerebrovascular diseases. Though scholars from many countries have spread research on the cerebral infarction in many directions and have made great progress, the effect isn't satisfactory on the whole. The reason lies in the difficulty of super early diagnosis, insensibility of the window time of nerve protective therapy, dissolution of thrombus and absence of special effective drugs. For a long time, both neurosurgeons and neurophysicians are bothered with this problem. So, study on cerebral infarction has been an important theme that needs a breakthrough in medical research up to now. MRS (magnetic resonance spectrospcopy) is a way that makes use of the Magnetic resonance phenomenon and the chemical shift effect to analyse a series of nucleus and its combinations. And now MRS is the only scatheless technology that can observe the variety of cells in vive and show the metabolism process directly, the application of MRS makes it possible for the study on cerebral infarction to reach the level of cells in vive. Recently, advanced countries have made great progress in MRS. But, this study is still at the beginning at home. The previous study showed that the chemical displacement of rat's brain was similar to humans? This experiment adopted rats for the animal models of focal cerebral isehemia and reperfusion. It was mimic the clinical pathological process of reperfusion in the animal model after the formation of thrombus in the-6-brain or the block of celebral arteries from the thrombus in organs otherthan the bIain at the different levels of blood glucose. By observing thevariety of MRS of the metabolism, we can prove the regularity of thecerebral metabolism When the rats made cerebral infarction were givenreperfusion and high glucose by variety of MRS and make a base for studyon the early diagnosis and the theraPy of cerebral infarction.Materials and methods: Nine male sprague dawley rats weighing230--270g were divided random1y into three grouPs and every group hasthIee rats. We chose modified Longa's intraluminal suture method to makeanima1 model. The first grouP is only used to make models of cerebralinfarction without further reperfusion. The second one is used to makemode1s of cerebral infarction-reperfusion. The rats of the third grouP aregiven 50% glucose 5gilig tWo hours after cerebral infarction-reperfusion.All the three groups are under the constan observation of MRS. We choseproton magnetic resonance spectYoscopy to analysis area under the wavesof NAA (N-Acety1aspartate), Lac (Lactate), Cho (Choline), Pcr/Cr(Ph o spho cre atine/Cre atin e), Glu/GAB A (Glutamate/Gama- amino butyri cacid) and compared the data of the accumulated areas under the waves.The eXPeriment data were managed statistically by analysis of varianceand 1east significant difference. A1l the rats were sacrificed and had theirbrain taken ollt just after MRS tests. We took random1y tWo rats out ofeach group for 2,3,5,-Tripheny-2H-Tetrazolium chloride (TTC) dye toconfirm the necrospot of infarction, the remainders were used to makepathological slices of Hematoxyln Eosin (ffo) dye.Results: This eXPeriment showed that the five waves of NAA, LAC,CHO, PCR/Cr,GlujGABA all have changes either under the conditions ofcerebral infarction or befOre and after the high-glucose was given. Theincreasing of Lac wave was the first sign. It increased l5 minutes after theischemia(p<0.0l). Later on the Lac wave kept stable at a higher level andreached to another peak after 60 minutes of ischemia. When the cerebral- 7 -ischemia was reperfused, the wave of Lac dropped in a short time (P<0.0l)but after a longer time (about 3 hours), it rose extreme... |
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