Effects Of Trauma On Soluble L-selectin And Cell Surface Expresion Of L-selectin

Objective: To examine the effects of trauma on changes in neutrophil L-selectin expression in rats and on the levels of soluble L-selectin in adults.Methods: The extent of the injury was calculated using an objective Injury Severity Score(ISS).Minor trauma was defined as ISS=1 to 8.moderate trauma as ISS=9 to 15 and major trauma as ISS>16. Levels of soluble L-selectin in adults with mechanical trauma were measured in the plasma, sampled concurrently , by ELISA. Blood sample was withdrawn from rat by cutting tail as minor trauma(equal to ISS=4),with fracture of femoral shaft as moderate trauma(equal to ISS=13), and with taking off the skin of neck and carotid puncture as major trauma. Neutrophil L-selectin expression in rats were analyzed by flow cytometry. Results: Neutrophil L-selectin expression in rats with minor trauma showed no change (p>0.05), with moderate trauma reaching peak levels in 4 hours and lasted 5,6 hours after injure, and early up-regulated,late down-regulated with major trauma. No significant changes in levels of soluble L-selectin in adults with minor trauma or moderate trauma were found at either stage.There were significant increase in 4 hours with major trauma compared with minor trauma or moderate trauma.These were down梤egulated in 6 hour after admission. Conclusion: These findings suggest that the increase of L-selectin may promote the adhesion of leucocytes to the venule wall and the inflammatory response and enhance the ability of anti-infection.The reduction of L-selectin may alleviate the adhesion reaction and SIRS.