Research On Esophageal Cancer Associated Gene-macrophage Migration Inhibition Factor Related Proteins 14

Esophageal cancer(EC) is one of the most common tumors of human beings, and China is with the highest EC incidence and mortality rate in the world. Epidemiology studies indicate that both environment and gene factors were the main cause of ECThe objective of this research is to search for the down-regulated genes in esophageal cancer.Methods The samples were collected from fresh tumor tissues and matched adjacent normal mucosa. DD-PCR (mRNA differential display-reverse transcription polymerase chain reaction) was performed with three pairs of esophageal carcinomas and adjacent histological normal mucosa. The interested cDNAs were analyzed by Northern blot and RT-PCR.Results: Using the 3' anchor primer and OPA3, DD-PCR showed a frequently down-regulated cDNA in the cancer tissues, but high expressed in normal tissues. By cloning and sequening, the cDNA was identified as the 3'-end of MRP14 (macrophage migration inhibition factor related proteins). Northern Blot and RT-PCR showed MRP14 was down-regulated in 6/6 and 10/12 BC tissues, respect ively.Conclusion: MRP14 was substantially down-regulated in esophageal cancer tissues.