The Study Of CD5～+B Cells In Peripheral Blood Of Patients With Hepatitis B

CD5+B cells, which develop from precursor B cells and are independent of bone marrow, are important subgroup of B cells. CD5+B cells can produce many autoantibodies which play important roles in auto-immune diseases. The recent research suggests that the numbers of CD5+B cell increase in peripheral blood of patients infected with HBV and HCV, and the more CD5+B cells exist, the worse the condition of patients will be .All these studies demonstrate that CD5+B cell may play important role in acute and chronic pathologic reactions in liver diseases and relative diseases. As there is a high morbidity of hepatitis B in China, it is valuable to study the effect of CD5+B cell on chronic liver disease and the relationship between CD5+B cells and patients condition. In order to investigate the changes of CD5 at genic level, the semi-quantitative RT- PCR method had been established to detect CD5 niRNA level in 50 patients suffered from acute ,cbronic,severe chronic HB and posthepatic cirrhosis. The ratioes of CD5 mRNA level of patients to housekeeping gene were 0.27±0.16, 0.76±0.18, 0.80±0.020 and 1.82±0.25, but the ratio of controls was 0.25±0.12. The results demonstrate that the level of CD5 nRNA increases gradually with the development of FIB. So CD5 mRNA transcription level could be regarded as the diagnosis marker for the severity. In this study, autoantibodies such as ACA, ANA, RF, anti-CaM, anti-CyPA and anti- LSP in 50 patients were detected by indrect ELISA. The results show that the percentage of CD5+B cell and CD5 mRNA are positively related to ACA and anti-CaM in chronic FIB, severe chronic FIB and posthepatic cirrhosis, but the percentage of CD5~B cell and CD5 niRNA of FIB patients has no relation with LSP. The results indicate that CD5~B cells play an important role in HE auto-immune reaction and long term process of FIB disease. The CD5+B cells in peripheral blood of 50 FIB patients were detected by imniunohistochemistry. Positive reaction rates were respectively (24.6±4.2)% in acute HE, (38.8±8.0)% in chronic HE, (44.4±8.4)% in chronic severe HE and (64.7 ± in posthepatic cirrhosis. The percentage in negative controls(n=10) is (23.0±3.1)%. There is no significant difference between acute FIB patients and controls, while CD5B cell postive percentages in other groups are higher than that of controls. The relativity analysis of percentage of CD5+B cell and liver function index indicate that the percentage of CD5+B cell in chronic KB and chronic severe HE patients have positive relations with ALT and TB, CD5+B cell percentage in posthepatic cirrhosis is positively related to ALT, AST, y -GT, TB and DB. The increase of CD5+B cell probably has close relation with liver function damage. Therefore, the detection of CD5+B cells maybe helpful to diagnose the process of HE and chronic liver disease.