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The Application Of The Multiple Factor Experiment To The Screen Of The Combinations Of The Attractants Of Rodenticidal Baits

Posted on:2002-08-05Degree:MasterType:Thesis
Country:ChinaCandidate:D S JiaFull Text:PDF
GTID:2144360032952373Subject:Epidemiology and Health Statistics
Abstract/Summary:PDF Full Text Request
Rodenticidal poison baits is the most ordinary means of rodent control, and the effects of rodent control of the poison baits lie on the palatability of those poison baits. The palatability of poison baits to rodent of different kinds in same and/or different area is varied, so that the trial of the palatability of those poison baits must be frequent. The reports on the combinations of the attractants of rodenticidal baits are usual, but the content of every kind of the attractants is uniquely appointed. Ever if the palatability of the result indicated that the poison baits combined by several attractants were better, it is unknown that whether the content of every kind of attractants was the best one and whether the combination was the most suitable. Li addition, the experimental rodents are captured from local area to laboratory for the normal choice feeding tests, or are replaced simply by rats or mice. It is difficult that certain amounts of same kind of rodents are captured from field. The results of the individually caged feeding tests hi laboratory can't completely present state that the local rodents feed naturally. The result of tests in which rodents are replaced by rats or mice still need be confirmed on field trial. To search the method more scientific and effective of the combinations of the attractants of rodenticidal baits, factorial experiment and the orthogonal design were applied to the choice feeding tests in field, by which the combinations of attractants of rodenticidal baits werescreened for the most suitable bait. The effect of rodent control of the most suitable bait is evaluated in field. This kind of test was reported previously.The field trial on screen of the combinations of attracts of rodenticidal baits by factorial experiment was conducted in the residential area and neighboring housebroken area located in eastern suburb of Nanjing. The area of the trial field is about 75 ,000m2, and the preponderant rodent is Rattus norvegicus. 36 testing baits poisoned with 0.005% bromadiolone were entirely combined by which sugar (A), peanut oil (B) and salt(C) were selected 3 levels of content and fishmeal (D) 2 levels, and were experimented in field for the field choice feeding tests with the control poison bait. Every poison bait was tested with 4 testing spots. The method of the field choice feeding tests is that every testing spot was laid together two bait trays, and two bait trays were filled 200g testing (control) poison bait and plain bait respectively according to the consumption of plain bait hi every bait tray in advance; the remainder of every bait tray was balanced for consumption of poison bait in 24h, then testing (control) poison bait and plain bait in every bait trays was supplemented. The consumption of baits in every bait trays was noted in 2 days. SPLM Chinese statistical analysis software was applied to analyze data, and the most suitable combination of the attractants was selected. The field trial of the effect of rodent control was conducted with the most suitable poison bait and control poison bait. The method of optimization of combinations is that according to the results of analysis of variance, the level that the variable K is maximal is selected to the factor that the differences among its levels is significant; the level that the variable K is second maximal is selected to the factor that the differences among its levels is no significant. By the results of analysis of variance, the differences among levels of sugar, salt and fishmeal were significant (P<0.05), but peanut oil no significant (P>0.05). So that the most suitable combination is A2BiC]Di, just 13th testing poison bait, which feeding coefficient is 1.94, oppositely the feeding coefficient of control poison bait 0.54. Adding fishmeal not onlydescended the palatability of testing poison baits, but also presented interaction with salt (P<0.05). There isn't second interaction between 3 attractants (P>0.05). By the field trial of rodent control with the baiting consumption method, the mortal...
Keywords/Search Tags:Rodent controlBaits, Attractant Screen, Palatability, Mortality Factorial experiment, The orthogonal design
PDF Full Text Request
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