Orthogonal Design Group Optimization By Animal Studies (jianpiyiqi Group Part) On The Uc Side

Purpose:To analyse the effect of the medicines in"the prescription to cure ulcerative colitis"whether is the major or secondary. And whether they have the benefit effect each other. Then we can estimate the best collocation of the prescription and we can remove the unnecessary medicine to optimize the prescription.Material and method:We use the SD rat to make the animal model. The reagent which used to make the model is (2, 4, 6– trinitrobenzen sulfonicacid, TNBS) and alcohol. It is a more used advanced method recently. My experiment plan is use an orthogonal design program to observe and analyse the medicines in"the prescription to cure ulcerative colitis"whether is the major or secondary. And find out whether they have the interaction each other. Then we can estimate the best collocation of the prescription and find the way to optimize the prescription.Results:After analyse the data of the experiment depend on orthogonal design, we find that the order of the medicines important degree is Dangshen,Atractylodes,Poria cocos,Licorice. We get the same conclusion in the data analysis both from gross specimen and Tissue section. There is some different in the data analysis. But we can find that there is some benefit interaction in the combination of each three medicines. And each combination make up with any tow of Dangshen,Atractylodes and Poria cocos has the same interaction. It is worth mentioning that the Licorice is not display any benefit interaction in the combination with any single medicine, but it display a benefit interaction in any combination with three medicines include it. It has a clear improve than the combination mixed up with the other tow. That is just the characteristic of the traditional Chinese medicine prescriptions.Conclusion:1. The important degree order of the group of strengthening the spleen and replenishing qi medicines in"the prescription to cure ulcerative colitis"is Dangshen,Atractylodes,Poria cocos,Licorice.2. There is some benefit interaction in the medicines of the group strengthening the spleen and replenishing qi in"the prescription to cure ulcerative colitis". It can improve the effect of treatment to the ulcerative colitis.3. The medicines in the group strengthening the spleen and replenishing qi of"the prescription to cure ulcerative colitis"is come from the Sijunzi decoction. It has been used in clinic for thousand years. Our experiment is also prove the prescription is reasonable. We can't remove any medicine from the prescription and optimize it.