Effects And Mechanism Of HRT On Bone Metabolize Of Perimanopause Women

Objective: To investigate the distribution of the common symptoms among postmenopausal (PMOP) women, to certificate the validity and rationality of the treatment of Hormone Replacement Treatment (l-IRT) to PMOP, to study the expression of MMP-2 and TIMP- I protein in osteoblast of SD rats and to assess the change of MMP-2 and TIMP-1 induced by 17-bata extradio (E2), progesterone (P), and to understand the mechanism of MMP-2 and TIMP-l in bone resorption. Methods: 1. A cross-sectional epidemiological study by questionnaire and a double- blind randomized placebo-controlled trial were adopted. 2. The expression and localization of the MMP-2 in cultured osteoblasts were demonstrated immunohistochemically. 3. The expression of MMP-2 and TI1MP-ImRNA in cultured osteoblast were assayed by RT-PCR after E and P were administered respectively. Results: 1. The prevalent clinical symptom in 1616 cases of both premenopause and postmenopause women investigated, aged 40-60 years (mean 46.2), was back pains (49.2%) last year. 2. 19 patients were treated with placebo as control, Fufuchun was administered as HRT to 16 patients for 30 days. 16 of 19 observed symptoms and their Kuppennan's values were significantly improved in HRT group than control. The Octeocalcin (OCN) and ALP in serum of HRT group increase significantly (p