| Background Psoriasis vulgaris(PV) is a chronic, inflammatory, hyperproliferation skin disease that affecting about 1%-3 % of the world population, but affecting 0.123 % Chinese, there were over 3,000,000 patients of psoriasis in China. Up to now, the pathogenesis of psoriasis remains elusive, genetic and environmental factors have been suggested as playing etiological role in the disease. As there is clear evidence from population and twin studies of a strong heritable component to the etiology, attention has recently focused on possible genetic determinants. Genome-wide linkage analyses have identified putative susceptibility loci on chromosomes 1 q, 2p, 4q, 6p, 8q, 16q, 17q and 20p. Recently, the study has mapped a major locus for psoriasis susceptibility to a 12cM region at chromosome 6p2l .3, this interval contains the 4Mb HLA. During the past over 20 yeas, several HLA antigens including A1, B13, B17, Cw4, Cw6 and A5 have been reported to increase the frequencies in patients with PV in Chinese. The introduction of polymerase chain reaction typing using sequence specific primers (PCR-SSP) have proved to be sensitive and specific in detecting HLA-DQA1 and-DQB1 al]eies. Objective To explore HLA-DQA 1 and DQB I alleles are associated with genetic susceptibility to psoriasis vulgaris (PV) in Chinese. Method Polymerase chain reaction-sequence specific primer (PCR-SSP) method was used to analyze the distribution of HLA-DQA1 and DQBI alleles among 189 patients with psoriasis and 273 healthy persons. Results ?HLA~DQA1*0104(OR=2.33, pO.OOO1154,p~ < 2.0 X i0~ ) DQA1*0201(OR=3.36, pO.0000000, p~, < 1.0 X 106 ) .~ DQBI*201(OR=1.64, p=O.O 192, p~>O.O5) and DQBI *0303(oR~4 .55, pO.O377, p~>O.O5) alleles were higher in PV than in controls. The allele of HLA~DQA1*0501(OR=0.30, P攡O.OOOOO39,P~<4.OX iO~) was significantly lower in PV than in controls. 〩LA- 4 DQAI*0104(QR=2.42, P=O昈OO1159~ ~ K 2.0 )< 1O~ Y DQAI*0201(0R3.74, p=O.0000000?p~< 1.0 X 106) DQB1*0201(OR=1.72, ~=O.Ol42?p~>O.OS) f~I~ DQB1*0303(OR=1.64,p=0.0240,p~.>0.05) alleles increased in type I PV than in heathy.The allelic frequency of DQA 1*0501 (0R0.32, p=O.00003 74,p~<4.O X 1 0~) was decreased in type I PV. The allele of DQAl*0501(OR=~0.27, p=O.0465, PL> 0.05) was decreased in type I PV. The HLA~DQB1*0501(QR=4.21, p=0.O349,p~ >0.05) was prevalent in type II. 瓾LA~DQA1*0104(OR=3.05, p=0.0004786, PC <5.0 x 1 o~I and DQA1 *020 1 (0R4.74, p~O.OOO0004, p<4.O X iO~) alleles were higher in the patients with faily history than in the control groups. HLA- DQA1*0301(OR=0.41,p=0.0159,p~>0.05) and DQAI*0501(OR=0.35,p=0.0154, p~>O.OS) alleles were lower in the patients with family history than in the controls. HLA~DQA1*0104(QR=2.08, pO.OO3128, PL K 0.05 ) ~. DQA1*0201(OR=2.90, p=0.0000084, p~< 8.0 X 10~) and DQB I *0303(0R1 .61, p=O.04l 6, p~ >0.05) increased in the patients without family history. The HLA~DQA1*0501(OR=0.30, p=O.0000084, p>Z6.OX iO~) alleles was significancely decreased in the patients without history. Conclusion ~IDHLA~DQA1*0104 and DQAI*0201 alleles may be the susceptible genes or it may have close linkage with...
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