Culture And Identification Of Neural Stem Cell From Rat Brain

Objective: To culture and identify neural stem cells (NSCs) . Methods: ㏕o culture neural stem cells by non-serum culture method with the presence of bFGF, EGF from hippocampus tissue and striatum tissue of PI rat brain and adult rat brain. (2) A quantitative count for neurospheres of primary culture of day 5 was employed to assess the difference of i\SCs between hippocampus and striatum of the same age and of different age rats. (3)NSCs and differentiated cells were identified by cell-specific markers immunohitochemically.Results: ells of primary and passage cultured continuously proliferated and formed neurospheres. ifferentiated cells were GFAP or NSE positive which suggested that they at least contained two kinds of cellseurons and astrocytes. oubing-labled staining showed that GFAP positive cells were much more than NSE positive cells which GFAP positive cells accounted for 73.8% and NSE positive cells for 26. 2%. ㏕he number of NSCs from PI rat brain were significantly more than that from adult rat brain(p<0. 01). But the NSC number between hippocampus and striatum of the same rat brain were not different (p>0. 05). Conclusions: There are neural stem cells in hippocampus andstriatum of PI and adult rat brain.