Polymorphism Of The VNTR Region In Intron 1 Of P53 And Discussing The Using In Lung Cancer

Objective Detect the polymorphism of the variable number of tandem repeats (VNTR) segment in intron 1 of the p53 gene of Chinese, make it useful to detect loss of heterozygosity ( LOH ) of the p53 gene. Investigate whether the heterozygous of the VNTR in intron 1 of the p53 increases lung cancer risk. Explore the relationship between LOH of the p53 gene and lung cancer.Methods Investigate blood samples from 150 unrelated healthy Chinese.* 57 patients of adenocarcinoma^ 46 patients of squamous cell carcinoma and matched pair tissue samples from 33 lung caner patients undergoing surgery. Before operation, none of the patients had received chemotherapy or radiotherapy. The amplification of the VNTR region was performed with PCR. The PCR products were run on 12% non-denaturing polyacrylamide gels.Results Of the 150 healthy Chinese, 102(68%)were found heterozygous in the VNTR region. Of the 103 lung cancer patients , 41 ( 71.9%) patients of adenocarcinoma and 31(67.4%) patients of squamous cell carcinoma show heterozygous. No significant difference was found between healths and patients. Of the 33 normal tissues, 29 (87.8%) were heterozygous; 6 of 29 (20.7%) revealed LOH; the LOH rate of p53 gene in small cell lung cancer(50%) is higher than in non-small cell lung cancer (12.5%) . No significant relationship was found out among the LOH of p53 gene and P-TNM stages and age of patients. Conclusion The VNTR segment in intron 1 of the p53 gene was highpolymorphic in Chinese, the rate of heterozygosity was 68%. In patients with lung cancer, LOH of VNTR segment in intron 1 of p53 gene can be detected.