| Objective To detect the frequency of MA on 18q, to investigate the function of MA during the progress of pathogenesis in sporadic colorectal cancer. Methods By PCR, polyacrylamide gel-electrophoresis and silver staining, MA was detected at 4 microsatellite loci on chromsome 18q in tumor tissues,adjacent tissues and normal colorectum tissues excised from 34 cases with sporadic colorectal cancer . Results 12 cases demonstrated MA at one or more loci ,the total frequency was 35.29% (12/34). The frequency of MA on D18S34, D18S473 . D18S487, D18S58 was 20.59% (7/34), 17.64% ( 6/34), 11.76% (4/34X 17.64% (6/34) , respectively. In only one patieftfr adjacent tissues , MA was detected at one selected locus. The freguency of MA of poorly differentiated carcinomas and mucoid carcinomas (56.25%, 9/16) was significantly higher than that of well differentiated ones(16.67%, 3/18) , and the frequency in right colon( 61.53%, 8/13) was significantly higher than that in left colorectum (19.05%, 4/21). MA-positive cases were youngerthan negative ones ; there were no statistic significance ( P>0.05) in the frequency of MA between male (38.64%,7/19) and female(33.33%, 5/15) . Conclusions The most common MA occurrence at the 4 loci might imply the existence of the potential genes related to the carci nogenesis of colorectal cancer. and provide a effective index for early diagnosis of colonrectal cancer. Between MA positive and negative cases , some events were found as follows: tendency towards younger patients (usually<50 years in age), more frequentcy in right colon and more frequency in poorly differentiated carcinomas.
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