Loss Of Heterozygosity And Microsatellite Instability On Chromosome 3p14 In Cervical Carcinoma

Objective:The pupose of this study was to determine the frequency of LOH and MSI on 3p14 in cervical carcinoma in order to facilitate the isolation of the candidate tumor suppressor genes associated with CC and discuss the possible relations between LOH and MSI, clinical stage and clinic-pathology. Methods:In this study we analyzed LOH and MSI in 41 cases of CC using the polymerase chain reaction for amplification with 2 polymorphic microsatellite repeats on chromosome 3p14. Results:25 of CC cases showed at least one loci of LOH.The loci of D3S1600 was 28%,the higher frequency of D3S1300 was 35%.The occourence of LOH showed correlation in different clinical stage and clinic-pathology. The frequency of MSI were 7.5% and 12.8% respectively. But no significant difference in statistical analysis. Conclusion:The results from this study taken together with others indicate that LOH on chromosome 3p14 are common in high grade of CC. These imply that LOH on 3p14 are the important determinants of clinical stage and clinicopathological characteristics of patients with CC.Tumor suppressor gene that play a role in cervical carcinoma may be located on the 3p14,likely at or near D3S1300,D3S1600 loci.