Study Of The Expression Of PTEN And P16 Gene In Vulvar Carcinoma

Objective To study the relationship between tumor suppressor gene PTEN and P16 and the generation and development of vulvar carcinomas.Methods By use of immunohistochemistry, the protein of PTEN and P16 were detected in 10 benign vulvar tissues and 25 primary vulvar carcinomas.Results All samples of benign vulvar tissues were found to express PTEN protein and P16 protein, low expression rate (60.00%) of PTEN and low expression rate (64.00%) of P16 were found in vulvar carcinomas .The protein positive expression rate of PTEN and P16 in vulvar carcinomas were significantly lower than that in benign vulvar tissues(P<0. 05).The expression level of PTEN protein was higher in well-differentiated group (Gl,77.78%) than that of less-differentiated group(G2. G3,14.29%), the expression level of P16 protein was also higher in well-differentiated group (Gl, 83. 33%) than that of less-differentiated group (G2. G3, 28. 57%). Moreover,the expression level of PTEN and P16 protein in stagel. II(68.42% and 73.68%)have higher tendency than that of stage III.IV(33.33% and 33.33%). There was no difference between the expression levels of different histological types and different ages.Conclusions The results suggest that loss of PTEN and P16 protein expression might play a role in the generation anddevelopment of vulvar carcinomas. Measurement of the expression of PTEN and P16 protein in patients' tumors might help in predicting prognosis, directing clinical treatment and providing theories for genetic therapy.