The Significance Of Fibroblast Growth Factor (FGF)2,7,10 Expression In Benign Prostate Hyperplasia Tissue

Benign prostate hyperplasia (BPH) is an extremely common prostate disease, occurring in more than 70% of men over the age of 60,and result in substaintial morbidity in this population, including complications such as urinary retention, renal impairment and infection. And yet the pathogenesis is still obscure. The expression of Fibroblast Growth Factor (FGF^ 7^ 10 in benign prostate hyperplasia and normal prostate tissue was studied by quantitive RT-PCR and by immunohistochemistry.Methods: Expression of FGF2 ^ FGF7 and FGF10 was evaluated by quantitative reverse-transcriptase polymerase chain reaction (PCR) techniques. Comparative analysis of expression of FGFs in BPII and NP. The localization and expression of FGF2 and FGF7 was evaluated by immunohistoehemieal technique.Results:1. RT-PCR: Expression of FGF2, FGF7 and FGF 10 was found in BPH and NP by quantitative reverse-transcriptase polymerase chain reaction (PCR) techniques. And expression of FGF2^ FGF7 and FGF 10 in BPH was high than in NP.2. Immunohistochemistry: To tetermine the localization and expression ofFGF2 and FGF7 in NP and BPH by immunohistochemistry with rabbit anti-FGF2 monoclonal antibodyand rabbit anti-FGF7 monoclonal antibody. We noted FGF2-positive and FGF7-positive cells were located in stromal cells and in epithelial cells of NP and BPH. The expression of stromal cells in BPH were higher than in NP.Conclusions: Overexpression of FGF2 FGF7 and FGF10 may play an important role in the abnormal cellular proliferation seen in benign prostatic hyperplasia.