Determination Of JingA In FXH And NaoQuTong Granules By RP-HPLC Method

PART 1(1) Determination of Total flavonoids in FXH by UV methodObjective: To establish a UV method for the assay of Total flavonoids in FXH.Method: Total flavonoids were determined by UV-spectrophotometry and the detective wavelength was set at 512nm. The chemical reference substance was rutin. The extract solvent was 95%EtOH.Result: The linearity was in the range of 0.0901-0.0451 mg, r=0.9998(n=5). The average recovery and relative standard devition respectively were 100.60% and l.l%(n=6).Conclusion: The method was found to be simple, rapid, highly accurate and sensitive. It can be used for determination of total flavonoids in FXH.(2) Determination of JingA in FXH by RP-HPLC Method.Objective: To establish a RP-HPLC method for the assay of JingA in FXH.Method: JingA is a effective component in FXH. It was determined by RP-HPLC with Hypersil ODSCi8(5um,4.6mmX 250mm); The mobile phase was ACN-H2O (33:67) and the detective wavelength was set at 212nm;The flow rate was 1.0ml per minute and the column's temperature was room temperature; The extract solvent was MeOH.Result: The linearity was in the range of 0.36-1.86ug, r=0.9995(n=5). The average recovery and relative standard devition respectively were 99.47% and 0.68%(n=6).Conclusion: The method was found to be simple, rapid, highly accurate and sensitive. It can be used for determination of JingA in FXH.PART 2Determination of Arctiin in Fructus arctii L. by RP-HPLC Method.Objective: To establish a RP-HPLC method for the assay of Arctiin in Fructus arctii L.Method: Arctiin is a effective component in Fructus arctii L.. It was determined by RP-HPLC with Hypersil ODS Ci8(5um,4.6mmX 250mm); The mobile phase was ACN-H2O (28:72) and the detective wavelength was set at 280nm; The flow rate was 1.0ml per minute and the column's temperature was room temperature; The extract solvent was MeOH.Result: The linearity was in the range of 1.58-7.90ug, r=0.9993(n=5). The average recovery was 99.84% and the CV% was 0.80%(n=3).Conclusion: The method was found to be simple, rapid, highly accurate and sensitive. It can be used for determination of Arctiin in Fructus arctii L..PART 3(1) Determination of JingA in NaoQuTong Granules by RP-HPLC Method.Objective: To establish a RP-HPLC method for the assay of JingA in NaoQuTong Granules.Method: JingA was determined by RP-HPLC with Hypersil CDS C18(5um,4.6mmX 250mm); The mobile phase was ACN-H2O (28:72) and the detective wavelength was set at 212nm;The flow rate was 1.0ml per minute and the column's temperature was room temperature; The extract solvent was MeOH.Result: The linearity was in the range of 0.922-8.298 jig, r=0.9997(n=5). The average recovery and relative standard devition respectively were 99.40% and 0.65% (n=9) .Conclusion: The method was found to be simple, rapid, highly accurate and sensitive. It can be used for the quantitative determination of NaoQuTong Granules.(2) Determination of Arctiin in NaoQuTong Granules by RP-HPLC Method.Objective: To establish a RP-HPLC method for the assay of Arctiin in NaoQuTong Granules.Method: Arctiin was determined by RP-HPLC with Hypersil ODS C 18(5jum,4.6mmX 250mm); The mobile phase was ACN-H2O (28:72) and the detective wavelength was set at 280nm; The flow rate was 1 .Oml per minute and the column's temperature was room temperature; The extract solvent was MeOH.Result: The linearity was in the range of 4.52-2.6ug, r=0.9998(n=5). The average recovery was 99.53% and the RSD% was 0.75% (n=9) .Conclusion: The method was found to be simple, rapid, highly accurate and sensitive. It can be used for the quantitative determination of NaoQuTong Granules.