The Expressions Of C-Met, VEGF And PDGF And Their Relations To Angiogenesis In Endometrial Cancer

Endonietrial cancer (EC) is one of three malicious tumors of female genital tract .In recent years,it's morbidity has a trend to increased. It threatens women's lives seriously. Studying it's mechanism in tumorigenesis and seeking for effective method in therapy have become important problems for study. Researches has indicated that the growth of tumor is dependent on angiogenesis. Tumor cells can secret many angiogenic factors that promote tumors' growth. So, antiangiogneic therapy has become very important in tumor therapy .In addition,angiogenesis is also mediated by proto-oncogene and tumor suppressor gene. Micro vessel density(MVD) is quantitative indicator of angiogenesis. Researches have indicated that vascular endothelial growth factor (VEGF), platelet-derived endothelial growth factor(PDGF) and c-Met, a proto-oncogene, play an important role in angiogenesis of tumors.But a few research about them in EC is reported in foreign journals and scarcely is reported in Chinese journals. The preasent study was to investigate the expressions of c-Met, VEGF and PDGF and their relations to angiogenesis in endometrial cancer(EC) so as to learn the mechanism of tumorigenesis in EC and to offer the theoretic basis to theantiangiogenic therapy of endometrial cancer.Materials and methods: Endometrial cancer specimens were obtained from 45 patients who were surgically treated from 1996.4 to 2000.4 in the third and the first affiliated hospital of zhengzhou university.A control group (15 cases) with normal endoinetrimn comprised specimens obtained from patients who underwent hysterectomy for uterine leiomyoma and intraepithelial carcinoma. All specimens were stained immunohistochemically for factor Vffl-related antigen. Three fields were selected for microvessel count in the most intense areas of neovascularization and the mean microvessels count were calculated. We also investigated the expressions of c-Met, VEGF and PDGF identified immunohistochemically with specific antibody and the relation between their expressions and the angiogenesis of EC. All experimental data were analysed with chi-square test or t test and processed by SPSSlO.O.There was a statistical significance when PO.05.Results: (1) MVD was significantly higher in EC than in normal endometrium. (18.36+9.34 vs 8.33+3.26, P<0.05). MVD was significantly higher in stage III and IV than in stage I and II. ( 26.75 + 10.51 vs 16.54 + 8.14, PO.05). MVD was significantly higher in grade 2and 3 than in grade l.( 22.77 + 9.24 vs 14.13 + 7.41, PO.05). It was also significantly higher in tumors with 1/2 mymometrial invasion than with 0.05).MVD were higher in c-Met-positive tumors than in c-Met-negative tumors(21.14+9.83 vs 13.31+5.82 P<0.05). (3) The positive expression rate of VEGF in EC was higher than in normal endometrium(P<0.05). The expression level of VEGF is higher in grades 2 and 3 than in grade 1 (PO.05). It was also higher in stages III and IV than in stage I and II (PO.05). There was no significant difference in VEGF expressions in different myometrial invasion(P>0.05). MVD in VEGF-positive tumors is significantly higher than in VEGF-negative tumors. (4)The positive expression rate of PDGF in EC was higher than in normal endometrium. There was no significant difference between the expressions of PDGF and the stage, grade and myometrial invasion(P>0.05). A higher MVD was noted in PDGF-positive tumors than in PDGF-negative tumors (P<0.05).Conclusion: (1) MVD was significantly higher in EC than in normal endometrium and with the development of EC MVD increased whic...