The Beneficial Effects Of Growth Hormone And 21-aminosteroid On Bacteria And Endotoxin Translocation In Obstructive Jaundiced Rats

The Protective Effects of Growth Hormone on the Heal Mucosa in Obstructive Jaundiced RatsObjective: To study the mechanism of bacteria and endotoxin translocation and the protecive effects of human growth hormone(GH) on the ileal mucosa in bile duct ligated rats.Methods: Thirty SD rats were randomly assigned to three groups.they were sham operated (SO,n=10),bile duct ligated (BDL,n=10) and bile duct ligated with growth hormone treated (BDL+GH,n=10). Two weeks after the operation all rats were sacrificed and the concentration of endotoxin in portal and systemic blood was determined;the bacteria of their mesenteric lymph nodes,liver specimens were cultured;the distal ileal mucosa was used for determination of insulin-like growth factor-I mRNA by RT-PCR and for measurement of morphological changes.Results: Treatment with GH significantly reduced bacteria and endotoxin translocation from the gut and ameliorated impairment of ileum mucosa;expression of IGF-I mRNA in ileal mucosa was decreased markedly after bile duct ligation and could be reversed by GH administration.Conclusion: Obstructive jaundice promoted bacteria and endotoxin translocation from the gut and could result in impairment of ileum mucosa. ;GH treatment showed a protective effect through up-regulation of IGF-I mRNA in ileum.