Enhancing The Efficacy Of Antitumor Drugs By Electroporation

Cell membrane is the crucial target of electromagnetic pulse field (EMPF). In is composed of bilayer phosphate and protein including liquid crystal and solid crystal,and has the nature of fluidity and permeability. The permeability barrier is withheld when the cell membrane is exposed to a short high voltage EMPF. The electroporation is the transient pore or permeable structure on the cell membrane that will be last for 20 milliseconds and will be closed at the end because of the fluidity. The electroporation ratio of the different kinds of cell exposed to the same EMPF is different,so do the same cell exposed to the different EMPF. Furthermore,the viability of cell and the kind of buffers also affect the ratio of poration. In this paper,Hela cell handled by EMPF with various voltages,times,capacity,is contrast put in with unhandled cell,the handled cell is found to have been electropored. The frequency,pores number,depth of poration are relative to the EMPF parameters. At the same time,the microvilli are damaged. Furthermore,the pore likes of volcanic vent and ejecta have been seen,then the best parameters of EMPF to Hela cell have been found. All above provide primary evidence for the electrochemotherapy of Hela cell.CTX is delivered into Hela cell by electroporation. By cell flow sorter and the stains of typannan,the life ratio is reduced very much,the apoptosis ratio and the death ratio are increase severely,and the cytotoxicity at the low pose is so tense as the high pose. By the stain of yading,the quintessential apoptosis cell with nuclear condensity and the flaw have been founded. Furthermore,the life ratio has nothing todo with the density of ami tumor drug when the density reaches somedegree.As a result of EMPF influence,the life ratio of cell handled only byEMPF is less low than the control cell' s.The subcutaneous xenograft of slSO tumor cell on the KM rats has been used for experiments evaluating the response of tumors to various therapies. The cure ratio and the partial response ratio of the rats theraped by ECT is more high than the rats theraped by drugs only or EMPF only. The control rats have no response. Results show severe necrosis and edema in nearly all the rats treated by the drug and the pulse. The group with only drug treatment did not show any significant edema and the tumor volume of the control group was found to increase more much,but the tumor volume regressed with drug in combination with electric field.Phlyctenes were observed at the sites of electrode contact with the skin,superficial leukonecrosis of some nodules was visible within the following 3 days,and scabs were observed a few days later,and the tumor volume decrease until regression. Separating the tumor from the body of rats. The tumor of rats treated by the drug and the pulse compared to the tumor of rats treated only with the drug or the pulse has little of vasculature,and the control has quite a few of vasculatures. Also,histopathological studies indicated that all the tumor cell was necrosis and the tissue from the tumor site showed complete absence of tumor cells when the tumor volume was complete regression . No tumor reappeared within 20 days.