A Study For Treatment Of Tumor By Anti-Cancer Drugs With Electroporation

Electroporation is believed to be the transient creation of micropores in lipid bilayer of cell membrane due to the effects of the electric pulses. It is a biophysics process, which can increase the permeation and conductance of cell membrane. This increase allows many molecules such as aqueous affinity molecules, virosomes, DNA, proteins and dye granules, etc, which can not pass through cell membrane in normal conditions, to pass through it.The electrochemotherapy(ECT) is a technology that uses electric pulse combined with medicament to treat cancer. The foundation of this scheme is to get electroporation in membrane by electric pulse, sequentially make it easier that medication comes into the cell to bring its effect into play and improve the effect at counteract cancer. Our team chose the S180 sarcoma cells and use the electric pulse produced by the LN-101 Gene Pulse Transfection Apparatus, combined with several anticancer drugs in a series of experiments and got a series results, which provide basis for the feasibility of the ECT.In this paper, there are four sections. In the first part, we have studied the improving drug's effect on the treatment of tumor by electric pulse. The result was estimated from the growth of the sarcoma, the histochemical observation, the observation from TEM and so on. In the second part, wehave studied the best electric pulse parameters and the best drug time of the electrochemotherapy to cure the S180 sarcoma of mice. The result indicated that within our presumed scale, there was some difference but no obvious difference when the electric pulse parameters changed. But let the drug time be 1min and 15min, the result was that the curative effect of the 1min-team was better than the 15min-team. In the third part, we calculated and analyzed the electric pulse and the distributing of the voltage by program MATLAB and computational method (FDM). The results show that the frequencies of electric pulse are at low part and the highest frequency is no more than 400Hz. In the fourth part, we drew a conclusion of the treatment of tumor by Anti-cancer drugs with electroporation and give some advices.