| Studies were conducted to determine the comparative effects of a -tocopherol (T) , y -T, 5 -T and n-tocopheryl-succinate (VES) on HepG2 cells growth and viability in vitro. The results showed that 6 -T and VES inhibit proliferation of HepG2 cells significantly. Gamma-T showed weak inhibition, and -0--T has no effects. With the exception of ^_-T, 8 -T and VES were effective in induction of apoptosis in HepG2 cells when tested within the range of their solubility, 10-200mg/L. Gamma -T showed effect only in concentrations higher than 100mg/L The proliferation of HepG2 cells were blocked in GO/G1 phase after treatment with 8 -T and VES as evaluated by flow cytometric analysis. This occurrence of apoptosis was associated with a cell cycle-specific mechanism. Delta-T and a. -TS decreased HepG2 cells growth and viability, and increase apoptotic propensity significantly. A dose-dependent of antiproliferative and induction apoptosis was found in HepG2 cell lines. The efficiency of four vitamin E analogues was in the order of 8 -T>VES> y -T> CL-T. The difference in nature and magnitude of the anticancer effects does not correlate with their reported relative antioxidant activity and might be due to minor differences in their structure important to their biological activities. These results suggest that 6 -T and VES could be promising anti-hepatoma agents.
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