The Experimental Research And Study On Dampness-heat Syndrome To Measure Heat And Dampness

OBJECTIVE:research the experimental parameter of dampness-heat syndrome to measuring heat and dampness,and observe the pathological change of dampness-heat syndrome by copying a dampness-heat syndrome animal model,to find the standard diagnosis and therapy for dampness-heat syndrome.METHODS:use normal Wistar Rats,lay out four groups,normal control group,dampness-heat syndrome model group,QingXiang powder treatment group,treatment control group. Make a dampness-heat syndrome model. HE staining method was used to observe intesting,spleen and liver's pathological changes. (1)Plasma ET and CGRP be detected by RIA method;(2)Bradford method to detect AQP2;(3)ELISA method to detect G-CSF;(4)colorimetric method to detect MMS and SH.RESULTS:(1) Many lymphocytes infiltrated their spleen and intesting,some of their spleen have a small vessel Dilation,the liver almost haven't any pathological changes;(2) The model group has a lower AQP2 level than normal control group,and makes a quick recovery after a treating by QingXiang powder.The dampness-heat syndrome model group has a higher ET level than normal control group,but has a lower CGRP level than it(p<0.01). The dampness-heat syndrome model group has a lower SH level than the normal control group,but has a higher G-CSF and MMS level on a significant of difference in statistics (p<0.01). All of the above difference wasbecame to common level after a week by QingXiang powder treatment,and the treatment control group has a higher positive rate of G-CSF.CONCLUSION:(l)AQP2's function is important to dampness,we can use AQP2 measure dampness whether dampness be heavy or slight. (2) Measure and weight heat was related to the feedback mechanism of vessel-center-vessel,. (3) Under the dampness-heat state,the variance of MMS,R-SH and G-CSF was related to dampness-heat and Bacterium infection colligation agents,this may represent the heaviness or slightness to the dampness-heat syndrome state of an illness in the pathological procedure.(4)the mechanism of QingXiang powder treat dampness-heat syndrome is related to it adjust the equilibrium of cells inner and outer body fluid,adjust proteolysis and protein synthesis,reinforce cells self-protective functions etc.