Studies On The Steroidal Chemical Constituents And Quantitative Determination Of β-Sitosterol Of Oldenlandia Diffusa(Willd.) Roxb.

Oldenlandia diffusa( Willd.)Roxb. is recorded in the <> for the first time .The herbal is a whole grass plant of Rubiaceae.Property,flavour and channel tropism:bitter and sweet in flavour,cool and cold in property,acting on the heart,liver,spleen channel.Effects:heat-clearing and detoxicating,heat-clearing and inducing dampness.In folks,this medicine can be used to treat commonly swollen and sore throat,cancer ... etc..The modern pharmacology expresses that the herbal medicine can be antibiosis,eliminate inflamation and anticarcinogenic,and P-Sitosterol is one of active constituents of Oldenlandia diffusa( Willd.)Roxb.. Objective:to study on extraction and separation and identified of the steroidal chemical constituents,and to establish the method of quantitative determination of P-Sitosterol in Oldenlandia diffusa(Willd.) Roxb.Methods:the constituents were gained by refluxed with CHC13 for three times,then were separated and puried by column chromatography with silica gel.The strutures were identified by mp. IR. NMR. MS. Quantitaty of P-Sitosterol in Oldenlandia diffusa(Willd. ) Roxb. was determined by TLC-scanning for the first time.Results:Two compounds were obtained from Oldenlandia diffusa(Willd. ) Roxb extraction and identified as Stigmasterol (A),P -Sitosterol (B). Quantitaties of P-Sitosterol in Oldenlandia diffusa(Willd.) Roxb. were 0.09-0.20% respectively.Conclusion:The method was highly sensitive reproducible for quantitative determination of P-Sitosterol. The method can be used for the quality control of Oldenlandia diffusa(Willd.) Roxb.. Two compounds can be gained from Oldenlandia diffusa(Willd.) Roxb and identified as Stigmasterol (A),0 -Sitosterol (B).