The Study Of Correlation Between Lactobacilli And Vaginal Infections In Pregnannt Women

Vaginosis is one of the most common genital infections among women during childbearing age. During perinatal period, increased level of estrogen stimulate the deposition of glycogen in the vaginal epithelium, the enviroment of the vagina is adapt to the growth of bacteria, so the incidence of vaginal infections is increased in pregnant women. Recently, many studies show that vaginal infections, particularly bacterial vaginosis, is linked to a number of pregnant complications, including spontaneous abortion, preterm delivery ,premature rupture of the membranes , amnionitis, postpartum endometritis, and so on. Lactobacillis are the predominant microorganisms in the vaginal microecosystem of healthy women, they play an important role in the maintenance of the vaginal ecosystem balance, protect the host agaist infection by pathogenic microorganisms. Many properties required to confer this protection have been identified,such as producing hydrogen peroxide ^ producing acid. Lots of clinical studies indicate that maintennance of lactobacillus-predominant vaginal flora will decrease the incidence of vaginal infections in pregnant women. There are some reports that the instillation of exogenous lactobacilli into the vagina result in decreasing the incidence of vaginal infections.However, in these studies, lactobacilli has usually been identified only to the genus level because of the technical difficulties in the species identification of Lactobacilli. It is uncertainly which lactobacilli species is adapt to cure and protect vaginal infections in pregnant women.In this study, according to clinical signs and Gram stain of vaginal fluidsmear, we investigate the correlation between lactobacilli and vaginalinfections, such as bacterial vaginosis, vulvovaginal candidiasis. Lactobacilli species are identified by use of biochemical method, acquire the distribution of lactobacillus in the vagina of pregnant women, compare ability of producing H2O2, decreasing the culture enviromment PH among four common lactobacilli species in the vagina of pregnant women. The aim of our study is to select lactobacilli species with beneficial properties for probiotic use in the vaginal tract of pregnant women.The result and conclusions are as followed:1 > In the health pregnant women, the presence of lactobacilli is same as the pregnant women infected vulvovaginal candidiasis , but it is obviously higher than that of bacterial vaginosis;2> The predominant Lactobacillus colonizing in the vagina of pregnant women are L. crispatus and L. acidophilus;3> The frequency of isolation for HiCh. positive Lactobacilli in vagina of health pregnant women is significantly higher than vaginas of BV> WC.4> Most clinical isolates of L. crispatus and L. acidophilus produce HiC^ and their ability of decreasing the culture enviromment PH is high.The study indicates that HaC^-positive Lactobacillus play an important role in prevention from vaginal infections hi pregnant women. As the predominant H2O2 positive Lactobacillus colonizing in the vagina of pregnant women , L. crispatus and L. acidophilus may be have advantages in the maintenance of normal microflora and prevention from vaginal infections in pregnant women. The study provide theorial foundations of that, either or both of these two lactobacillis may be used to prevent and cure vaginal infections in pregnant women in the future.