Studies On The Relationship Between Ankylosing Spondylitis And HLA-B₂₇ And Its Alleles Distribution

Objective: To investigate the relationship between Ankylosing Spondylitis and HLA-B27 and its alleles Distribution as well. Methods: 43 cases of Ankylosing Spondylitis and 45 cases of controls had been tested on their HLA-B27 antigen with the methods of complement dependent microcytotoxity assay (CDMA), ploymerase chain reaction-sequence specific primers (PCR-SSP) and flow cytometer. Results: The frequencies of positive HLA-B27 in the Ankylosing Spondylitis group were apparently higher than those in the control group (P<0.01), while there was no distinctive difference in the frequency among the three methods(P>0.05). No other HLA-B27 alleles but B*2704(87.8%) and B*2705(12.2%) were found in Ankylosing Spondylitis group. Conclusions: the relationship between Ankylosing Spondylitis and HLA-B27 is certain. Only B* 2704(87.8%) and B*2705(12.2%) of HLA-B27 alleles exist in Ankylosing Spondylitis group. The positive frequencies of HLA-B27 show no difference among the three methods, but each has its own characteristics.