Immunohistochemical Study Of Matrix Metalloproteinase-1 And Matrix Metalloproteinase-3 In Psoriasis

Objective To investigate the expression and localization of matrix metalloproteinase-1,3 in lesional and non-lesional skin of patients with psoriasis and normal huaman skin , in addition we analysis the correlation between the expression of MMP-l,MMP-3 and psoriasis area and severity index (PASI), to explore their role in the pathogenesis of psoriasisMethods We chosed 25 patients with psoriasis who were final diagnosis in clinical and pathology. The severity of psoriasis was assessed using the PASI scoring system. S-P immunohistological staining was used to detect the expression of MMP-1 ,MMP-3 in 25 cases of lesional skin and non-lesional skin of patients with psoriasis and 10 cases of normal human skin .Results The positive rates of MMP-1 and MMP-3 in lesional and non-lesional skin of patients with psoriasis and normal huaman skin were 21/25(25%), 14/25 (56%), 0; 16/25 (64%), 0, 0, respectively . MMP-1 and MMP-3 found in lesional skin of patients with psoriasis was significantly higher than that in normal human skin (P<0.05) . The expression of MMP-1 found in lesional and non-lesional skin of psoriasis was higher than that in normal skin(P<0.05). The expression of MMP-3 was only observed in lesional skin of patients with psoriasis . MMP-1 and MMP-3 were mainly found in the basal layers and superdermis layers. The expression rate of MMP-1 and MMP-3 were gradually increased with PASI .MMP-1 and MMP-3 levels in psoriasis had a strong correlation with PASI (ri=0.771, r2=0.763, PO.05).Conclusion MMP-1 and MMP-3 are found in psoriasis ,which may play an important role in the pathogenesis of psoriasis , which may contribute to intensive infiltration of local inflammatory cell.