Study On The Mechanism Of Nm23-H1 Inhibiting Human Breast Cancer Cell

Objective: To study on the mechanism of human breast cancer cell inhibited by nm23-H1 and provide an evidence for the treatment of mammary cancer with nm23-H1 gene.Methods: The pcDNA3.1-NMH1, a recombinant expression vector which contain human nm23-Hl gene and eukaryotic expression vector was established, and both the breast cancer cell line(MCF-7S) and the human umbilical vein endothelial cell line(ECV-304) were transfected with the pcDNA3.1-NMHl through lipofectamine regent, and the positive transfectant of the MCF-7S and the ECV-304 obtained by 6418 was collected to be amply cultured in vitro and comparatively studied the effects of the pcDNA3.1-NMH1 on the proliferation, the plating efficiency and the motility. In addition, in the ECV-304, the transcription of nm23-Hl gene examined by RT-PCR and the NM23-H1/NDPK-A determined by means of immunohistochemistry as well as the morphology changes observed by the transmission electron microscope(TEM) were comparatively studied.Results: Compared with the control cells, the effects of the pcDNA3.1-NMHl on the proliferation, the plating efficiency and the motility of both the MCF-7S and the ECV-304 were significantly inhibited, which were prolonged the time of the log-growth period, shorted the motility distance and decreased the growth rate or the plating efficiency. In addition, in the ECV-304, the expression of nm23-Hl mRNA and its protein products NM23-H1/NDPK-A were positive and mainly presented in the cell plasm besides the mitochondria were swelling and reduced in number and appeared the inner cista deformation or even disappearance. Conclusions: the mechanism of human breast cancer cell inhibited by nm23-H1 wassignificantly correlated to the inhibited effects on the proliferation, the plating efficiency and the motility of the tumor cell as well as that of the vascular endothelial cell. The effective site of the angiogenesis inhibited by nm23-H1 might be in the mitochondria of the vascular endothelial cell, which provide a target for further study.