| PrefaceThe tumor and tuberculosis is the two kinds of disease that threats human health. Both of them happens relate with the immunity lowli-head. Many studies suggested that T lymph cell plays an important role in immune responsion. T lymph cell what we always call thymus dependent lymphocyte, plays an important role on body cellular and humoral immune induction.The main effect of anti - carcinoma is cellular immune. Many studies showed T lymph cell is the key during body immune reaction. Tumor may form if body immune function decrease or immune monitor and clearance cant find it out. On the other hand , the transcription activity of T lymph cells rDNA may decrease, caused by inhibiting cy-tokin in the serum of patients with tumor. The result is T lymph cell protein synthesis and body immune function inhibited.The major immune reaction to tuberculosis is cellular immune. It is a specific immune reaction of T lymph cell by the effect of antigen. Tuberculosis, a kind of intra - cellular parasite, may cause cell - induced immune reaction when it invade the body. Then, whether the infected body is sick depends a lot on this bodyfc immune function.We used KL - immune analysis system, which has advanced image analysis, to analyze the development of rDNA transcription activi-ty, at the area nucleus formed. This system synthesizes modern oncology, immunology, cytobiology and computer image analysis technique. Quantitive analyzed Ag - NORs in T lymph cell of the patients with malignant tumor and pulmonary tuberculosis, which reflect the rDNA transcription activity. The index is the ratio between the surface areas of silver - dyed and the nucleus.The purpose of this research is to find out whether the index has difference between the normal person and the patients with tumor and pulmonary tuberculosis, and its value in the assistant diagnosis.Objects and Methods1. Objects( 1) Patients with TumorThey were the patients of the 1st and 2nd affiliated hospital of China Medical University. All the 27 patients were definitely diagnosed of malignant tumor in histology and had not got radiation therapy or chemical therapy.(2) Patients with Pulmonary TuberculosisAll the 27 patients were chosen from Chest Hospital of Shenyang. They were definitely diagnosed by X - ray and sputum smear.(3) Healthy peopleThe 27 healthy people come from Center for Disease Control And Prevention of Shenyang. The patients and those in control group match with one other in age ( 5 years) , sex and race.2. Epidemiological InvestigationWe investigated those whose blood samples were gathered in the morning by filling up well - designed tables looking up medical recordand patient reviewing.3. Experiment Methods ( 1) cells culture(2) cells isolation ( 3 ) making slices(4) Ag - straining(5) Analysis by computer4. Statistical AnalysisStatistical soft we used is SPSS11.5. All the data were indicated by XS. Comparation of data among groups is made by q - test, x2- a~ nalysis.ResultsThe value of I. S% is much lower in patients with tumor and tuberculosis than in health people. And obvious differences were found between them (P < 0.001).There were obvious differences between I. S% of tumor patients and those of tuberculosis patients ( P <0.05).No differences were found in both different types of tumor ( P > 0.05 ) and different type of tuberculosis ( P > 0. 05 ) .DiscussionImmune system is mainly line of defence of anti - carcinoma in body. On the effect of cause mutation factor, There are about 107 ~ 8cells happened gene saltation everyday. Some cells can translate into Tumor cell. Normal immune monitor function can distinguish and clearthese cells. Tumor can't happens. The malignancy often occurs in body immune function decrease or comparatively decrease. T lymph cell is the mainly cell in the body that participate anti - carcinoma immune responsory. So, though monitor the patients function of T lymph cell to reflect the positive and development statue o... |
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