| ObjectiveFlumorph is one of the fungicides against cucumber downy ( Pseudoperonospora cubeusis) , brassica downy mildew ( Poronospora parasitica) , grapedowny mildew ( Plasmopora viticola) and tomato late blight ( Phytophthora infestans) with the perfect effect of the prevention and treatment at the concentration of 100 - 200mg/litre. It has a broad application range and its close relationship to people is osculation. In the toxicological studies of the flumorph, the results of Ames test and the mouse micro - nuclear test are negative, and the sub -chronic toxicological study showed that flumorph aroused abnormity of the liver function and anemia of the rats when flumorph with purity of 95% was used. In this study, the Chinese hamster lung cells (CHL and V79) were used to detect the chromosome aberration and gene mutation induced by flumorph. It is important for the security of human health and the environmental security to further study the muta-genesis of the flumorph.Materials and Methods1. The test cells: V79 cells were bought from Biochemistry and Cell Biology of CAS and stored in fluid nitrogen after being selected by 1% HAT culture medium for 7 days. CHL cells were bought from National Safety and Evaluation Center of the New Drug (Shenyang).2. Drug: Flumorph ( C21 H22 FNO4, molecular weight: 271. 4 ) with purity of 98% was supplied by Shenyang Chemical Industry Research Institute.3. Methods3. 1 The toxicity of flumorph to V79 cells and the half inhibition concentration (IC50 ) of CHL cells treated by flumorph were examined in the absence of a metabolic activation system.3.2 The HPGRT gene mutation examination was detected with different dose levels of flumorph in the absence and presence of the metabolic activation system. The V79 cell was treated for 2 hours at the dose levels of 500,100,20 and4g/ml. The negative and the positive control were designed in the same time. 6 -TG( 10g/ml) was used to select the mutant cell clones. The cell clone forming rate and the mutant clone forming rate were measured and the mutant rate wasanalyzed.3. 3 The chromosome aberrance examination was performed in the absent and present of the metabolic activation system. The cells were treated for 24 and 48 hours respectively at the dose levels of 500, 250,125 and 62. 5g/ml, and the negative and the positive control were designed in the same time. The glide sample was prepared and dyed routinely by the Giemsa and G - Banding techniques. 100 kary-gotypes in different doses were observed with each dying technique. The ratios of the chromosome aberration were analyzed statistically.Result1. The result of the cell toxicityIn the absence of the metabolic activation system, the V79 cell clone forming rates treated by flumorph were 7.3%,40.4%,68.0%. 92.1% and 98.9% in the dose levels of 500,100,20,4 and 0. 8g/ ml accordingly, and the half inhibition concentration (IC50) of CHL cell treated by flumorph was 250g/ml.2. The result of the HGPRT gene mutation test in V79 cells induced by flumorphIn the absence and the presence of the metabolic activation system , the rates of the mutant clones from V79 cells treated by flumorph in the dose level of 500,100,20 and 4g/ml were similar to that of the negative control, which means that flumorph could not induce the increasing of the rates of the mutant clones of V79 cells.3. The result of the chromosome aberrance examination of CHL cellsThe ratios of the chromosome aberration of V79 cells treated by flumorph for 24 and 48 hours were less than 5% in the absence of the metabolic activation system, and there was no difference between the ratios of the test groups and that of the negative control; The ratios of the chromosome aberration treated for 24 and 48 hours by flumorph were more than 5% in the presence of the metabolic activation system in every dose level, and the ratios of the chromosome aberration went up with the increasing of the dose levels, which showed clear dose -response relations. The results were c...
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