The Expression And Significance Of Neuron Specific Enolase And S-100β After Cerebral Ischemia Reperfusion In Rats

Objective: To observe the expression and significance of neuron-specific enolase (NSE) and S-100 β in the cerebral tissue after reperfusion of focal cerebral ischemia in rats; To explore the effect of Inosine on hypoxic-ischemic brain damage.Methods: The reperfusion of focal cerebral ischemia models were established with SD rats, which were divided into controlled group (saline solution was injected intraperitoneally) and treatment group (Inosine was injected intraperitoneally). Each group was divided into eight subgroups which consisted of 4 rats. Neurological grade evaluation was used to examine the behavioral recovery on these rats. At 2h, 6h, 12h, ld, 2d, 3d, 7d, 14d after reperfusion of MCA, immunohistochemical technique was used to investigate the dynamic changes of NSE and S-100 β in cerebral tissue.Results: (1)There was improvement in the score of neurologic grade in Inosine treatment group at 7d and 14d after reperfusion when compared with controlled group. (2) NSE: The expression of NSE in cerebral cortex and striatum was low in sham-operate group. In controlled group, the peak expression of NSE occurred at 12h after reperfusion and its expression decreased gradually (ld-7d). At 14d it decreased to controlled level, in treatment group, the change of NSE was similar to the controlled group. The use of Inosine could increase the expression of NSE. In the two groups, the expression of NSE in cerebral cortex was higher than that in striatum. (3)S-100 β : The expression of S-100 β in cerebral cortex and striatum was low in sham-operate group. In controlled group, the expression of S-100 P increased at 12h and continued to increase from 1d to 3d. Thepeak expression occurred at 3d, it decreased at 7d, and to controlled group level at 14d. In treatment group, the change of S-100 β was similar to that of the control group. The use of Inosine could increase the expression of S-100 β .Compared with NSE, the time of peak expression of S-100 β was later. Conclusion: The expression of NSE and S-100β increased significantly in the cerebral tissue after reperfusion of focal cerebral ischemia; It is suggested that Inosine could protect against hypoxic-ischemic brain damage, whose role might be reflected by increasing the expression of NSE and S-100 β.Postgraduate: Wang Yue (Specialty of Neurology)Directed by Guo Yunliang...