The Effect Of Bcl-2 Antisense Oligonucleotide On Sensitivity To Cisplatin Treatment In Bladder Cancer Cells

Objective: To study the effect of antisense oligodeoxyribonucleotide(ASODN) to apoptotic suppress gene bcl-2 on sensitivity to cisplatin treatment in T24 cells. Methods: Cultured cells were divided into 7 groups: antisense ODN, sense ODN, antisense ODN+cisplatin, sense ODN+cisplatin, cisplatin,DOTAP and control. Sense and antisense ODN were transfected into T24 cells by DOTAP. The expression of bcl-2 gene was examined by immunocytochemistry and RT-PCR, and cell growth by MTT uptake. Apoptosis was identified by flow cytometry. Results: The bcl-2 expression of cancer cell in antisense ODN group was obviously downregulated. IC50 of cisplatin in antisense ODN group , 2.05±0.09 μg/ml, was significantly decreased compared to DOTAP group,3.62±0.15 μg/ml, sense ODN group, 3.81±0.16 μg/ml, and control group, 3.90±0.21 μg/ml. Apoptosis ratio of cells treated by both cisplatin and antisense ODN was greatly lncreased compared to other groups. Conclusion: Antisense oligodeoxyribonucleotide to bcl-2 can significantly decrease bcl-2mRNA, inhabit the expression of bcl-2 in T24 cells,and sensitize T24 cells to cisplatin .