| Objective To prepare a desired specific immunoadsorbent for anti-AChR antibodies in myasthenia gravis patients. Methods 10% cellulose beads activated by epichlorohydrin were prepared as carriers by suspension regeneration. As ligands, L-tryptophan and synthesized polypeptide of AChR at residues 67~76 were immobilized on cellulose beads to make immunoadsorbent. The influence of immobilizing conditions on immobilizing quantity was observed in detail. The adsorbents were reacted with patients' serum which contained anti-AChR antibodies at 37oC for 3 minitues with shaking. Before and after the reaction, the optical densities of the antibodies were determined respectively and adsorption capacities were calculated. Results The optimal conditions of polypeptide immobilization are 37oC, pH9.7 and 20 hours. The highest adsorption capacity of polypeptide adsorbent for anti-AChR antibody is 40.33%±2.29%, followed by L-tryptophan adsorbent with22.35%±1.47%. the statistical difference was significant (p<0.05). Conclusion Temperature, pH and time have direct influence on polypeptide immobilization. There is difference in adsorption capacities between the two adsorbents. The adsorbent withcellulose as carrier and polypeptide as ligand, which is of high affinity with antibody, is an ideal immunoadsorbent in specific immunotherapy of myasthenia gravis.
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