Effect Of HSH On The Intestine Tissue Ischemia/reperfusion Injury Of Rat After Resuscitation From Hemorrhagic Shock

inJury of rat after resuscitahon from hemorrhagic shockAb s tra ctOb j ectivef To observe whether hypertonic solution chloridehydroxyethyl starch 40 in j ection (HSH ) exerts any protective effecton the intestine tissue damage of rat after resuscitation fromhemorrhagic shock and intratracheal lipopolysaccharid e (LP S )Methods f Fifty-four SD rats were randomly divided intogroupC,group RL and group HSH.Each group was divided intothree groups according to the time (hours ) afterresu scitation(groupCl, groupC2, groupC4, group RL l, group RL2,group RL',group HSHl, group HSHz, group HSH#).Hemorrhagicshock and intratracheal LPS animal model was prepared. The leve1 sof superoxide dismutase (SOD ), molondialdehyde (MDA ) andmyeloperoxidase (MP O ) were determined .Neutrophil L-selectinexpression was measured. Samples of intestine were removed forhist ol ogi c an alysis.Results f 1 h after the resuscitation,significantly increased MDAlevels and reduced SOD levels were detected in group RL andgroup HSH. 2h after the resuscitation,MDA levels began to declineand SOD levels rise in group HSH.But there was no obvious changein group RL. 4h after the resuscitation,there was statisticallysignificant difference between group RL and group HSH aboutMDA and SOD levels (P<0.05 ) .Neutrophil L-selectin expressionand MPO levels in intestine tissue were significantly lower ingroup HSH than those in group RL at all times. There was a slightand statistically not significant elevation of MPO levels in groupHSH compared with group C. The histologic analysis showed that intestine injury slighter in group HSH than that in group RL. Conclusion: HSH can effectively supress neutrophil L-selectin expression .reduce oxygen free radicals levels and neutrophil infiltration in intestine,decrease tissue ischemia/reperfusion injury.