Quality Control Studies On Traditional Chinese Medicine Resina Draconis

The traditional Chinese medicine Resina Draconis is extracted in bark and xylem with fat of Dracaena cochinchinenesis (Lour.) S.C.Chen. How do different extraction technology impact the quality of Resina Draconis? To compare four kinds of extraction methods such as Reflux, Soxhlet method, Ultrasonic extraction and Cryogenic expansion with those sold in Yunnan and Hainan. They are identified by TLC and Ultra-violet spectrophotometry. A HPLC method of the quantitative determination of Loureirin B has been developed. Six kinds of Resina Draconis are identical basically in TLC and UV. The components of six kinds of Resina Draconis are identical basically, but there are some differences estimated by loureirin C. There are a high quantitation of loureirin B in production extracted by Reflux, Soxhlet method and Cryogenic expansion and a low quantitation extracted by Ultrasonic extraction. The quantitation in production extracted by Cryogenic expansion method is higher than traditional method. The quantity of loureirin B in all brands is lower than the limit of quality standard. The powder extracted by Cryogenic expansion method is very loose, but the stability of it is poor and the cost is higher. So the new extraction method, Cryogenic expansion, is not worth spreading. The ratio of Ultrasonic extraction is lower than that of other extraction methods. So the approach is not suitable to extract Resina Draconis. The traditional technology is reasonable for producing Resina Draconis.We find a problem that there is a big impurity peak near the peak of Loureirin B in quality analysis of different extraction technology by HPLC. It interferes the determination of Loureirin B seriously. This phenomenon has not been reported.In order to confirm the discover, we analyse five brands of Resina Draconis. The problem exists in every brand. The impurity compound is isolated and identified by phytochemistry. The compound is Loureirin A. The structure of LoureirinA is very close with that of Loureirin B, therefore it is extraordinarily difficult to separate Loureirin A and Loureirin B by normal HPLC. Optimizing conditions of HPLC, we measure the quantitations of Loureirin A and Loureirin B and find the quantitation of Loureirin A is 5 times as much as that of Loureirin B. A HPLC method of the quantitative determination of Loureirin A and Loureirin B in different brands of Resina Draconis has been developed. The relativity of Loureirin A and Loureirin B is very good, so the quantitation of Loureirin A is used in common quality control of Resina Draconis. It is necessary to emend the quality standard. Analyze Resina Draconis's extraction in petroleum ether by GC-MS, we find chief components of it are aromatic compounds and fatty acid compounds. Unsaturated fatty acid is more in fatty acid. The content of unsaturated fatty acid is 30% of extraction in petroleum ether. The activity of unsaturated fatty acid is thought as lowering blood fat and anti-tumor, but unsaturated fatty acid in Resina Draconis will be studied. To reveal active site of Resina Draconis and pharmacology activity, we undertake phytochemistry works and pharmacology research. We study activities of antiinflammation, hemostasia and anti-congestion and anti-fungi about Resina Draconis, part of acetic ether and part of butyl alcohol, adopting ripe models of pharmacology, and discovere pharmacology activity of part of butyl alcohol of Resina Draconis is very high, especially hemostasia activity and anti-congestion in body. NIRDRS cluster analysis is used to distinguish drug powder of different brands of Resina Draconis. The yunnan dragon's blood and guangxi dragon's blood extracted from Dracaena cochinchinenesis(Lour.)S.C.Chen and hainan dragon's blood extracted from Dracaena angustifolia Roxbfl Ind. Ed are rather different. While yunnan dragon's blood and guangxi dragon's blood are essentially identical. So NIRDRS cluster analysis is used to identify Resina Draconis. We develop a new approach, UV scanning feature value cluster analysis and...