Preparation Of Nanoparticles Containing Resina Draconis And Its Effect On The Skin Wound Healing

Resina Draconis is processed by Dracaena cochinchinensis (Lour.) S. C. Chen’s resin, it contains anthoxanthin and aetherolea as main composition. Resina Draconis has effection on dissipate blood stasis, analgesic therapy, stop bleeding, wound healing. The dissolubility of Resina Draconis is little in water, it is hard to be absorbed, and the bioavailability is low. So, if we can establish a new form of Resina Draconis which has good dissolubility, it will be very helpful in elevating bioavailability, and create a possibility to make better use of it.Nanoparticle drug delivery system is composed by drug or drug and material, its particle diameter is 1-1000nm, and these systems are quite active in pharmaceutics in recent years. Nanoparticle is much smaller than minimal human capillary vessel(4000nm) and red blood corpuscle (6000nm-9000nm), so they can reach every spot of human body by blood freely. There is two kind of nanoparticle in pharmaceutics:nanoparticle drug with or without carrier. Nanoparticle drug with carrier is a nanoparticle drug delivery system which is produced by drug and drug carrier material,drug’s absorption can be raised by carrier,and the system also has cellular affinity,ability of drug release delay, and ability of target, They can raise drug therapeutic effect and cut down side effect and toxicity. Nanoparticle drug is nanoparticle which is produced by drug directly. It’s effective to make the drug which is hard to dissolve in water into nanoparticle in order to raise the absorption, and biological availability can be raised too. Compare to nanoparticle drug with carrier, drug loading capacity of nanoparticle drug is higher.According to the characteristic of nanoliposomes(NL) which has good drug transport ability across cell, we start the research of NL first. We choose octadecanoic acid as lipid material, produce NL of Resina Draconis by solvent diffusion method. Particle size and zeta potential are investigated by zetasizer, the morphology of NL is investigated by TEM. We choose loureirin A as target, establish the technology of testing content of Resina Draconis in Resina Draconis NL by HPLC, calculate the drug encapsulation efficiency and drug loading capacity of Resina Draconis NL. Result shows that the 1 mg-ml-1 Resina Draconis NL(Resina Draconis: octadecanoic=1:1) we produce, whose particle size is 29.4nm, zeta potential is-20.2±1.9mV, morphology is globular, The linear range of loureirin A’s concentration is 0.135-5.4μg·ml-1, Y=33.694X+1.5898 (r=0.9999). The concentration of loureirin A is 1.43 pμg·ml-1, and the average drug encapsulation efficiency was 89.59%.Because the content of loureirin A in Resina Draconis NL is low, and the drug loading ability of NL is only about 5%, so we choose to produce Resina Draconis nanoparticle to raise the quantity of Resina Draconis in nanoparticle. We produce Resina Draconis nanoparticle by solvent diffusion method, investigate particle size and zeta potential of Resina Draconis nanoparticle by zetasizer, investigate the morphology of it by TEM. We choose loureirin A as target, establish the technology of testing content of Resina Draconis in Resina Draconis nanoparticle by HPLC, calculate the drug loading capacity of Resina Draconis nanoparticle. We evaluate the drug release profile in vitro by dialysis bag. Result shows that the 1 mg·ml-1 Resina Draconis nanoparticle we produce, whose particle size is 101.7nm, zeta potential is-19.9±1.6mV, morphology is globular, The linear range of loureirin A’s concentration is 0.135-5.4μg·ml-1, Y= 33.694X+1.5898 (r=0.9999). The concentration of loureirin A is 2.87 μg·ml-1. The drug release profile in vitro shows that compare to the suspension of Resina Draconis powder, liquid of Resina Draconis nanoparticle release 68% of drug, and the suspension of Resina Draconis powder release 40% only. It’s obviously that Resina Draconis nanoparticle has a good sense of drug release promoting.Above all the basic research, we produce Resina Draconis gel for external use. We choose 1%Carbomer 934 as basic gel, and produce gel of Resina Draconis nanoparticle, gel of Resina Draconis powder, gel of Resina Draconis alcoholic solution. We investigate the flowability, glutinosity and appearance of them. The result shows that gel of Resina Draconis nanoparticle and gel of Resina Draconis powder have low flowability and high glutinosity, gel of Resina Draconis alcoholic solution has low flowability and low glutinosity. The drug release profile in vitro shows that compare to the gel of Resina Draconis powder, gel of Resina Draconis nanoparticle release 83% of drug in 72h, and the gel of Resina Draconis powder release 52% only. It’s obviously that gel of Resina Draconis nanoparticle has a good sense of drug release promoting. Compare to the gel of Resina Draconis alcoholic solution, gel of Resina Draconis nanoparticle release 55% of drug in 4h, and the gel of Resina Draconis alcoholic solution release 76%. It shows that gel of Resina Draconis nanoparticle has a good sense of drug delayed release effect.We build animal skin wound model by cutting square skin in back of rat, use gel of yunnanbaiyao powder as comparison, evaluate the wound healing effect of Resina Draconis gel by eye’s observation, photo shooting and wound measuring, observe the slice of HE staining skin by electron microscope. The result shows that all three kind of Resina Draconis gel can shorten the time of wound healing, and the gel of Resina Draconis nanoparticle has best effect. In the beginning of research (3d,6d), the healing rate of Resina Draconis alcoholic solution gel is much higher than other groups, but in the later period (14d,20d), the group of Resina Draconis nanoparticle gel do better. Only in the group of Resina Draconis nanoparticle gel, rat’s wound skin totally healed in 20 days. According to the observation by electron microscope, we find many blood capillary in each group’s skin sample. It proves that all skins are active.