| Objective To investigate the mechanism of acupuncture on cerebral ischemic vascular diseases and appropriate time for acupuncture.Methods The focal cerebral ischemia rats model are made by middle cerebral artery occlusion(MCAO) with nylon thread embolus .We observe the improgress of nerve signs in the acute and chronic ischemic stage after electroacupuncture, detect the change of nitric oxide(NO),endothelin(ET),super-oxide dismutase(SOD),MDA of blood.We also detect the expressions of bcl-2 and bax which are neuronal apoptosis related genes. Results The results indicate that electroacupuncture can accelerate the disappearance of pathological nerve sign. The recovery at the group of acupuncture in acute stage of cerebral ischemia rats is better than that at the group of acupuncture in chronic stage . Electroacupuncture can decline the amount of NO, and decrease the poisonous effects on focal cerebral ischemia rats. Electroacupuncture also can reverse the rising trend of ET in the blood, thus improve the flow of blood in brain and protect further damage of the neurons. Electroacupuncture can improve serum SOD activation and reduce MDA content notably of focal cerebral ischemia rats,thus suppress production of free radical and delay neuronal cell death .Electroacupuncture can lead the expression of Bcl-2 protein and suppress Bax protein , then suppress the apoptosis of neurons.Conclusion The experiments have announced that electroacupuncture affected MCAO rats in acute and chronic stage from molecularbiology,cytology,histology and other levels.The results shows that the effects of acupuncture in acute stage is better than that in chronic stage.lt suggests that clinical acupuncture for cerebral ischemic stroke should begin from acute stage.
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