The Study On The Effect Of The Expressions Of Estrogen Receptor MRNA And Type â…  Collagen MRNA In Rats With Experimental Osteoporosis With Jianguling

Osteoporosis is a systemic skeletal disease characterized by low bone mass and microarchitectural deterioration of bone tissues, with a consequent increase in the bone fragility and the susceptibility to the fracture. The wrinklies, the edlies and postmenopausal women suffered from osteoporosis that may lead to the compressive fractures of lumbar vertebrae or the distal radial fractures ,the proximal femoral fractures and the superior humeral fractures ,which affect the existent qualities of the patients to a great extent. The fractures occurred lumbar vertebrae. And it was legion and fast that the studies on osteoporosis which breakthrough were prodigious from dose of calcium to the remedy of gene. Currently, studies of western medicine indicated that the HRT was the best way to OP. There was a controversial aspect of risk of cancer to patients yet. Because of its less poisonous side effect, the traditional Chinese medicine more and more was paid attention to. This experiment investigated the mechanism of JIANGULING on osteoporosis in the molecule level.To investigate the molecular links of the treatment with JIANGULING on postmenopausal osteoporosis in vivo, adult ovariectomy rats were used in this study as the model of postmenopausal osteoporosis. The numbers of osteoblast in the bone tissues were analyzed using HE staining; Estrogen mRNA and type I collagen mRNA expressions in bone tissues of ovariectomy (OVX), ovariectomy with diethylstilbestrol, ovariectomy with JIANGULING and ovariectomy with GUSHUKANG rats were analyzed at mRNA level using Tissue In Situ Hybridization (TISH). The results showed that the expressions of both ERmRNA and COLLA I mRNA were higher than those of VOX in the bone tissues(p<0.01). The numbers of osteoblasts of OVX-JIANGULING were more than the those of OVX (p<0.01).The tissues in TISH showed that ERmRNA and COLLA I mRNA were mainly expressed in osteoblasts. These datum indicated JIANGULING played an important role called " estrogen-like " , which prompted bone formation by regulating ERmRNA and COLLA I mRNA.