An Experimental Study On The Effect Of Qianggubao On The Metabolism Of Bone Collagen In Diabetic Osteoporosis Rats

Objective: To research the status of metabolism of bone collagen in the diabetic osteoporosis rats and the effect of Qianggubao on it, for the objective to probe into the pathogenesis of DOP and Qianggubao to the DOP preventing and controlling mechanismin the hans of metabolism of bone collagen.Methods: After the adaptation for one week, from 47 Sprague-Dawley rats (three months of age, and female and male to be half and half), 8 rats (half male and half female) were chosen at random according to sex as normal control group. Last 39 were established by in-troperitoneal injection of compounds 2.5% fresh Alloxan solution with the physiological saline according to 120mg/kg avoirdupois after to fast to be unable to restrain the water for 12 hours, and repeated it in the next day. To maintain the lives of rats, All rats from to make the DM mold to complete the latter 24 hours hind legs thigh hypodermic injection persistent effect insulin 0. 4U/100g avoirdupois as basic remedy , Once every other day. Making afterward mold 5 day the vein to pick the blood to measure the blood-glucose (BG), after 7 days, 9 days duplicates one time respectively, whose blood sugar value 3 times all greater than 16. 7mmol/L is successful DM mold. After 7 weeks, normal group and model grouprats whole body bone mineral density, (BMD) were examineed by X- ray bone density measuring apparatus examine with animal's software PRODIGY full-automatic pair, the rats levels of BMD lower to 2. 5 standard deviation than normal group were considered as the DOP rats, then taking 36 successful DOP mold rats to divide into the B DM model group, the aminoguandine-treated group(treaded with Xiaokewan+AlPhaD3), Qianggubao group, each group of each 12, female and male are half and half. All were provided normal feed . Rats of normal control group and DM model group were filled stomach lml/lOOmg avoirdupois with water, According to body surface area converting formulae between human and rat, the rats of Qianggubao group were filled stomach with with Qianggubao lml/lOOmg avoirdupois (herbs 4g/lml) and the rats of aminoguandine-treated group were feeded with the suspension liquid of Xiaokewan Tablets+AIPhaD3 liquor. lml/lOOmg avoirdupois (Xiaokewan 0. 75g/kg*d, AlPha D30. 05Hg/kg?d) . After fourteen weeks, all rats had a fast for 24 hours. Rats were drawing blood by means of Beheads , and detectived IL-6, TNF- a ? IGF-K PICP^ ICTP in its blood serum. The left shankbones were dipped in 4% polyformaldehyde in order to investigated type I collagen by immunohistochemical detection, and the right thighbones were used for examining bone collagen AGEs and bone collagen in every garm bones.Results: I After 14 weeks, compares with normal control group, in modeled group, The bone collagen AGEs value extremely remarkably rising (P<0.01 ), IL-6, TNF- a , the ICTP value notably risin g (P<0. 05); IGF-1, PICP, the shinbone each gram bone bone collagen content notably very much reduces (P<0. 01); and by bone collagen organization immunohistochemical dyes the observation demonstration bone collagen content remarkable reduction(P <0.01), and the collagen is tiny, sparse, disruption, arrangement ataxia. Compares with the model group, In Qianggubao group, The bone collagen AGEs value extremely remarkably reduces (P <0.01), IL-6, TNF- a , ICTP value obviously reduces (P<0.05); IGF-1, PICP, the shinbone each gram bone bone collagen content extremely markedly increase (P<0. 01); Bone collagen organization by bone collagen organization immunohistochemical dyes can see bone collagen content obviously increase(P<0. 01), also the collagen compact, arrangement neat. Compares with normal control group, in Qianggubao group, bone collagen AGEs, IL-6, TNF- a , ICTP value, IGF-1, PICP, the bone collagen content does not have the obvious difference(P >0. 05), the comprehensive curative effect surpasses the comparison western medicine.Conculsion: (l)Bone collagen of the DOP model rats is formated and reduced,resolves and accelerates, the metabolism presents and shoulders the balance state, the content of the bone collagen reduces; There is AGEs that is produced and accumulated excessivly in the bone collagen , cause the bone collagen fibre structure to be unusual , so that influence bone collagen metabolism and bone metabolism; (2)Organize cytokine factor level , suppress the forming of bone collagen AGEs and/or bone collagen AGEs removing a large number of savings with Qianggubao adjustable bone of strong bone treasure, thus improve bone collagen metabolism microenvironment of rats of DOP model effectively , improve the quantity and structure of bone collagen to unusually change, resume bone collagen of rats of DOP model to unusually metabolize. (3) Qianggubao directing or the indirecting control function to the bone collagen metabolism is it preventing and controling DOP an important biological mechanism.