The Expression And Relation Of P14～(ARF), P53, Mdm2 And E2F-1 Protein In Cutaneous Squamous Cell Carcinoma

Objectives: The present study aims to investigate the expression of p14ARF and together with p53, Mdm2 and E2F-1 status in 56 different histopathological Cutaneous Squamous Cell Carcinoma (CSCC), and to provide the information about any possible effect of all four proteins in the pathogenesis of CSCC.Methods: Immunohistochemical examination was performed on formalin fixed, paraffin embedded tissue sections of 56 different histopathological CSCC, using antibodies against p14ARF, p53, Mdm2 and E2F-1 proteins.Results: (1) The lack of p14ARF expression was observed in 19(33.9%) of 56 CSCC, and with the grade of CSCC increasing the lack of p14ARF expression was also increased (P < 0.05). (2) The incidence of p53, Mdm2 or E2F-1 expression was 78.6% (44/56), 39.3% (22/56) and 57.1% (32/56) respectively in CSCC, and there was a positive correlation between E2F-1 expression and the grade of CSCC (P<0.05).(3) There was a positive correlation between E2F-1 expression and Mdm2 expression (P<0.05). With the grade of CSCC increasing the number of aberrations of these protein expression was also increased (P<0.05). (4) There was no significant different of p14ARF or p53 expression between different age or sunlight exposure area groups.Conclusions: The aberrant expression of p14ARF, p53, Mdm2 and E2F-1 proteins played an important role in the pathogenesis and development of CSCC. The higher the rates of these protein aberrant expressions were, the more malignancy CSCC was, which was helpful for the diagnosis of CSCC. Inactivation of p14ARF or E2F-1 overexpression might serve as the indicator of malignancy potential in pathological diagnosis of CSCC, and Mdm2 expression was in accordance with E2F-1 expression.