Study On The Roles Of HPV16/18-E6, P53 And MDM2 Proteins In The Pathogenesis Of Human Laryngeal Carcinoma

[Objective]To determine the expressions of human papillomavirus type 16/18-E6 (HPV16/18-E6), p53 and Murine double minute-2(MDM2) proteins in different human laryngeal lesions and their relationship with the development of laryngeal squamous cell carcinoma(LSCC). [Methods]The expressions of HPV16/18-E6, p53 and MDM2 proteins were detected by the imrnuno- histochemical method in 119 specimens with different human laryngeal lesions, and their correlations with clinicopathological factors were statistically analyzed. [Results]The expressions of HPV16/18-E6, p53 and MDM2 were significantly different among the vocal cord polypus, the precancerous larynx and the LSCC(P<0.05). In LSCC, the positive rates of HPV16/18-E6 and MDM2 protein increased significantly from histopathological grade I to gradeâ…¢(P<0.05). The positive rates of HPV16/18-E6 protein were positively correlated with the clinical staging and metastasis of LSCC (P<0.05). The expression of p53 was positively correlated with the expression of HPV16/18-E6 and MDM2 in LSCC(P<0.05). [Conclusion]Inactivation of the p53 pathway and over- expression of HPV16/18-E6 and MDM2 may contribute to the development of LSCC.