The Experimental Study On CpG-ODN Improving The Immune Function

Objective: With umbilic blood from the mature newborns, to screen CpG-ODN, which can markly boost up the immunity response, from some designed CpG-ODNs; to compare the effect of CpG-ODN on the lymphopoiesis of umbilic blood from the premature, mature newborns and the peripheral blood from the adults; to observe the infulence of CpG-ODN on the immune function of the pregnant mices and their newborns, the immune-inhibited and aged mices.Methods: Five kinds of CpG-ODN were designed according to the different designing principles, the best CpG-ODN was screened by the tetrazolium salt assay (MTT), to observe the proliferative reaction on the peripheral blood monocyte cell from the premature, mature neonates and the adults; The recognized CpG1826 was used to immune the pregnant (20 μ g/mice), immune-inhibited (20 μ g/mice) and aged (20 μ g/mice or 30 μ g/mice) mices; Serum antibody (IgG) levels of the pregnant mices and their newborns, immune-inhibited mices and aged mices were determined by ELISA; The pathological slices from the spleen of mices were made up (dyed with HE) to observe the splenic cellularity under the microscope.Results: It was confirmed that CpG-ODN with 5'-GGTGCATCGATGCAGGG GGG-3 had evident immunity effect on the adults and mature newborns, and had no effect on the premature newborns. CpG1826 increased serum IgG concentration of the normal adult, immune-inhibited and aged mices, and did not influence the IgG levels of the pregnant mices and their neonates. within the dose 20 u g/mice used in this study, no side-effect such as neonates with intrauterine growth retardation (IUGR) exited.Conclusions: ① MTT assay is an ideal experimental method for screening CpG-ODN; ② Umbilical blood from the mature newborns can be used as an experimental material to screen CpG-ODN; ③ CpG-ODN with 5' -GGTGCA TCGATGCAGGGGGG-3 has a proliferative effect on the mature newborns and theadults, but no influence on the premature neonates; ④ with the dose 20 μ g/mice used in this study, the effect and side-effect (such as IUGR) of CpG1826 do not occur in the pregnant mices and their neonatal mices; ⑤ CpG1826 can improve the humoural immunity of normal adult, immune-inhibited and aged mices. It is shown that CpG-ODN as a new immunity adjuvant has a largely explored value and widely applied foreground.