| Objectives: To examine the distribution and infiltrating density of dendritic cells (DC) and T lymphocytes in different hyperplastic phases of breast intraductal epithelial cell carcinomatous change, and provide academic basis for clinical immunotheraphy.Methods: Immunohistochemistry for SI 00 protein, HLA-DR, CD la, and CD45RO was performed in 147 routinely paraffin embedded specimens including 16 cases of normal breast (Normal group), 20 cases of intraductal hyperplasia (IDC group), 29 cases of atypical intraductal hyperplasia (AIDH group), 9 cases of intraductal carcinoma and 6 cases of early infiltrating ductal carcinoma (DCIS group), and 67 cases of infiltrating ductal carcinoma (IDC group). The positive expression rates and infiltrating densities of S100+DC, HLA-DR+DC, CDla+DC (three parameters labeling DC), and CD45RO+T lymphocytes were investigated and compared with clinico-pathological parameters. In addition, the correlation among three parameters labeling DC and its relationship to CD45RO+T lymphocytes were analyzed. Results: 1. Positive expression rates of DC and T lymphocytes in different hyperplasticphases of breast intraductal epithelial cell carcinomatous change The positive expression rates of S100+DC in Normal, IDH, AIDH, DCIS, and IDC group were 32.25%, 40.00%, 44.83%, 53.33%, and 77.61% respectively. Accordingly, the positive expression rates of HLA-DR+DC were 43.75%, 70.00%, 51.72%, 100.00%, and 97.01% respectively. The positive expression rates ofCDla+DC were 0, 20.00%, 37.93%, 46.67%, and 56.72% respectively. The positive expression rates of CD45RO+T lymphocytes were 87.50%, 95.00%, 89.66%, 100.00%, and 100.00% respectively. The rates of S100+DC and CDla+DC in IDC group were significantly higher than those of the other four groups(P<0.05 for all). The rates of HLA-DR+DC in IDC and DCIS group were significantly higher than those of the other three groups (PO.05 for all). Combined expression rates of three parameters labeling DC were the highest in DCIS group (33.33%) and IDC group(55.22%). Contrast to the noncancer samples, all the cancer samples express at least one of the labeling positive DC in this study. There was no significant difference among the groups in the positive expression rates of CD45RO+T lymphocytes. 2. Infiltrating density of DC and T lymphocytes in different hyperplastic phases ofbreast intraductal epithelial cell carcinomatous changeInfiltrating densities of S100+DC in Normal, IDH, AIDH, DCIS, and IDC group were 1.19 + 0.41, 1.05+0.58, 1.13 + 0.70, 2.27+2.27, and 4.89 + 7.14 cells/mm2 respectively. Accordingly, infiltrating densities of HLA-DR+DC were 8.62 + 11.54, 6.52 + 3.89, 7.42 + 4.87, 12.40+16.40, and 20.70+18.52 cells/mm2 respectively. Infiltrating densities of CDla+DC were 0, 0.94+0.26, 1.25+0.54, 1.09+0.24, and 3.29 +4.37 cells/mm2 respectively. Infiltrating densities of CD45RO+T lymphocytes were 14.73 +13.59, 36.25+25.38, 49.07+27.49, 71.90+25.50, and 92.16 +66.78 cells/mm2 respectively. All infiltrating densities of three parameters labeling DC in IDC group were significantly higher than those in the other four groups(P<0.05 for all). Infiltrating densities of CD45RO+T lymphocytes were significantly higher in DCIS group and IDC group than those of the other three groups(P<0.05 for all), but there was no significant difference between two groups.3. Correlation among three parameters labeling DC and their relationship to CD45RO+T lymphocytesPositive correlations among infiltrating densities of three parameters labeling DC were showed significantly (P<0.001 for all), especially correlation between HLA-DR+DC and CD1a+DC was the highest. Positive correlations between infiltrating densities of three parameters labeling DC and CD45RO+T lymphocytes were showed significantly (PO.001 for all).4. Correlation between the expression of three parameters labeling DC and clinico-pathological parameters1) Infiltrating densities of S100+DC and HLA-DR+DC adjacent to the nest were significantly higher than those within the ne...
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