Effect Of Ambroxol On Expression Of Nitric Oxide Synthase In Rats With COPD

[Objective] To observe the effect of ambroxol on nitric oxide synthase in rats withCOPD and discuss the mechanism of the preventive and therapeutic effect of ambroxol on COPD.(Methods] 32 male Wistar rats were randomly divided into three groups.Thenormal group (A,n=8) ,The model group (B,n=12) and the ambroxol treated gro up (C,n=12) .Ratsin group B and group C were established by intratracheal instill ation of lipopolysaccharide twice and exposed to cigarette smoke.The group C wa s treated with ambroxol sincethe first day.Pulmonary and bronchial morphological changes were observed by light and electron microscopy .The quantitative patholo gical examination by image analysis wasalso performed.The expression of inducibl e NOS (iNOS) and endothelial NOS (eNOS) inpulmonary tissue was observe d by immunohistochemistry technique.(Results] Compared with group A, group B presented significant differences in PaCO2. PaO2. MLI and MAN [ (53±4) mmHg vs (41 ±3.5) mmHg, (80. 5±8.6) mmHg vs (98.4 ± 2.9) mmHg, (92±10) urn vs (52±3) um, (268 ± 15 ) /mm2 vs (410±27) /mm2 (P<0.01) ]; The expression of eNOS and iNOS were also markedly elevated (P<0.01). In comparison with group B , group C present ed significant differences in blood gas analysis [PCO, (48±3mmHg vs (53±4) mmHg, P02 (89.2 ± 7.3) mmHg vs (80.5 ± 8.6) mmHg) (P<0.01)]. MLK MAN were significantly different[ (83 ± 8) urn vs (92 ± 10) urn (P<0.05) , (291 ± 10 ) /mm2 vs (268±15) /mm2 (P<0.01) .The expression of eNOS were decreased (P<0.01), while the expression of iNOS were increased (P<0.01) .[Conclusion] Smoking can induce over expression of iNOS and weak expre ssion of eNOS in pulmonary tissue.The former might result in lung injury and COPD. Ambroxol can prevent the development of COPD in rats.Postgraduate: HAN WeiZhong (Respiratory speciality) Directed by: Prof. QIN XiaoMeiAssistant Prof. CHENG ZhaoZhong...