| Objectives: Proliferative vitreoretinopathy(PVR) is a common cause of blindness clinically,and is also the major cause of failure of retinal detachment surgery. The contract and traction of the proliferative membrane make the retinal tears which had been occluded successfully open again , bring new tears and cause the retinal redetachment. The treatmet of surgery,which does great harm to the issues of eyes and has difficulty in solve the problem of relapse,is not an ideal way to PVR. The optical function is not satisfactory though the retinal reattachment is successfully.The research of pharmacological treatment of PVR becomes a prospective direction for it,but thorough study the detailed application project and clinical employment evidence are scarce.We explored the effect of Danshen and trifluoperazine,which have the different inhibitory mechanisms , on experimental PVR in vivo and in vitro.Methods:In vitro: The retinal pigment epithelials (RPEs)(the 4th passage),which was separated alive,identified by immunohistochemistry and grow well, were inoculated in 96-well cultivative plate by 2×104/ml ,0.2ml/well.The RPEs were divided into 3 groups: IH764-3(31.7umol/L,15.85 umol/L,6.34 umol/L), trifluoperazine (20umol/L,15umol/L,10umol/L)and control group,6 wells/group.The MTTs of each group were measured on the time of 24,48,72,96,120 hours after pharmacological cultivation, and were compared with the controlled group,then the inhibitory rate was got.The data were analysed with the statistics software,then the IC50 doses were got to conduct the combined experiments.The process above was repeated twice with the examination of histology according to the corresponded dosage.In vivo: 51 rabbits were divided into 8 groups by random: 10/A~C group.One eye were selected for experiment. Another 10 eyes were selected as D group(the control group).The PVR model was created by the injection of RPE cells (2.5×105 /ml) to the cavity of vitreous.At the same time,group A were injected trifluoperazine (0.81mg/ml)0.1ml;Group B were injected IH764-3 (1mg/ml) 0.1ml; Group C were injected trifluoperazine (0.81mg/ml)0.05ml and IH764-3(1mg/ml)0.05ml ;Group D were injected PBS 0.1ml as control.All the eyes were observed by indirect ophthalmoscopy,slit-lampbiomicroscopy,and the situation of vitreous clouding and retinal detachment were recorded by B ultrasound and fundus camera on the time of 1st, 3rd, 5th, 7th, 14th, 21st, 28th days after the operation.Eyes were enucleated and examined by the light microscope 28days after the operation.There are 7 rabbits in each group of E ~G group, One eye were selected for experiment randomly. Group H was composed by the other eyes.The drugs corresponding to those of A ~D were injected shortly after 0.1ml PBS was injected. The eyes were examined by indirect ophthalmoscopy, electroretinogram(EOG) and light microscope at the time of 1st, 3rd, 5th, 7th, 14th, 21st, 28th day after operation.An eye was randomized to be enuleated and examined by light microscopes.Half of the eye which was enuleated on the 28th day were examined by light microscopes,and the other half part was examined by electronic microscopes.Results:In vitro: IH764-3 and trifluoperazine both have a significant inhibitory effect to RPE cells in the relationship of dose-effect and time-effect.It's The IC50 of IH764-3 was 15.22umol/L.The IC50 of trifluoperazine was 18.09umol/L.The inhibitory rate of combined drugs was 87.23±2.95%>75%(P<0.01),There was significantly cooperated effect to RPE cells during the application of IH764-3and trifluoperazine.Nontoxiceffect change was observed.In vivo: Group A~D: control group: retinal detachment appeared 3 days after operation.All had retinal detachment 7 days after RPE injection.Group of trifluoperazine:there was 2 eyes had the retinal detachment, 2 grade, and no change was observed during the experiment.IH764-3 group and the combined group:1 case of detachment took place, 2 grade.There was no change until the end of the research.Examinatio... |
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