| Objective:This experiment through the production of rabbit traumatic proliferative vitreoretinopathy model,to observe the effect of Sanqi injection on expression of transforming growth factor-?(TGF-?)and hepatocyte growth factor(HGF)in the retina of rabbits with traumatic proliferative vitreoretinopathy.In order to investigate the preventive and therapeutic effects of Sanqi on traumatic proliferative vitreoretinopathy in rabbits.Methods:A traumatic PVR was made by injecting scleral penetrating wounds combined with platelet-rich plasma in the vitreous cavity.The animals were divided into blank group,model group,positive control group,low dose group of Sanqi and high dose group of Sanqi treatment group.Different doses of Sanqi suspension were given for intragastric administration for 28 consecutive days.The positive control group was given intravitreous injection of daunomycine intravitreally.The expression of transforming growth factor-?(TGF-?)and hepatocyte growth factor(HGF)in rabbit retina was analyzed by immunohistochemistry.The expression were detected and analyzed.Result:(1)The number of ocular proliferative stages in each group: On the 1st day after administration,there was no statistically significant difference between the groups(P=0.296);after the 7th day of administration,there was a statistically significant difference between the groups(P<0.01),and the comparison between the model group and the blank group was statistically significant(P<0.01).The PVR scores of the positive control group and the high dose group of Sanqi were lower than the model group(P<0.01).The comparison between the model group and the positive control group was not statistically significant(P>0.05).There was no significant difference between the high dose group and the positive control group(P>0.05).(2)The cross-sectional area of fundus proliferating membranes in each group: The cross-sectional area of the fundus proliferating membrane in the rabbits of the model group was significantly higher than that in the normal group(P<0.01);the positive control group and the high dose of Sanqi had a significant difference with the model group.Statistical significance(P<0.01),but there was no significant difference between the low dose group and the model group(P>0.05).(3)The expression of transforming growth factor-?(TGF-?)in the retina of each group: compared with the blank group,the content of TGF-? protein in the retina of the model group increased significantly,with statistical significance(P<0.01).Compared with the model group,the levels of TGF-? protein in the positive control group and the high dose group of Sanqi decreased significantly(P<0.01),and the TGF-? protein content in the low dose group of Sanqi was also decreased.(4)The expression of hepatocyte growth factor(HGF)in the retina of each group: compared with the blank group,the HGF protein level in the retina of the model group was significantly increased,with statistical significance(P<0.01).Compared with the model group,there was no significant difference in the HGF protein content in the low dose group of Sanqi(P>0.05).The HGF protein content in the positive control group and the high dose group of Sanqi decreased significantly,with statistical significance(P<0.01).Conclusion:In this study,rabbit traumatic PVR models were successfully made by scleral penetrating injury combined with injection of platelet-rich plasma in the vitreous cavity.The study found that Sanqi can reduce the degree of fundus hyperplasia and the formation of fundus proliferative membrane in traumatic PVR rabbits,and at the same time,it can reduce the expression of TGF-? and HGF in the retina,thus preventing and treating traumatic PVR. |
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